Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells' ozone response
M. Nuhkat, M. Brosche, S. Stoelzle-Feix, P. Dietrich, R. Hedrich, M. Roelfsema, and H. Kollist. New Phytol, 232 (4):
1692-1702(2021)Nuhkat, Maris
Brosche, Mikael
Stoelzle-Feix, Sonja
Dietrich, Petra
Hedrich, Rainer
Roelfsema, M Rob G
Kollist, Hannes
eng
Research Support, Non-U.S. Gov't
England
2021/09/06
New Phytol. 2021 Nov;232(4):1692-1702. doi: 10.1111/nph.17711. Epub 2021 Sep 23..
DOI: 10.1111/nph.17711
Abstract
Plant stress signalling involves bursts of reactive oxygen species (ROS), which can be mimicked by the application of acute pulses of ozone. Such ozone-pulses inhibit photosynthesis and trigger stomatal closure in a few minutes, but the signalling that underlies these responses remains largely unknown. We measured changes in Arabidopsis thaliana gas exchange after treatment with acute pulses of ozone and set up a system for simultaneous measurement of membrane potential and cytosolic calcium with the fluorescent reporter R-GECO1. We show that within 1 min, prior to stomatal closure, O(3) triggered a drop in whole-plant CO(2) uptake. Within this early phase, O(3) pulses (200-1000 ppb) elicited simultaneous membrane depolarization and cytosolic calcium increase, whereas these pulses had no long-term effect on either stomatal conductance or photosynthesis. In contrast, pulses of 5000 ppb O(3) induced cell death, systemic Ca(2+) signals and an irreversible drop in stomatal conductance and photosynthetic capacity. We conclude that mesophyll cells respond to ozone in a few seconds by distinct pattern of plasma membrane depolarizations accompanied by an increase in the cytosolic calcium ion (Ca(2+) ) level. These responses became systemic only at very high ozone concentrations. Thus, plants have rapid mechanism to sense and discriminate the strength of ozone signals.
Nuhkat, Maris
Brosche, Mikael
Stoelzle-Feix, Sonja
Dietrich, Petra
Hedrich, Rainer
Roelfsema, M Rob G
Kollist, Hannes
eng
Research Support, Non-U.S. Gov't
England
2021/09/06
New Phytol. 2021 Nov;232(4):1692-1702. doi: 10.1111/nph.17711. Epub 2021 Sep 23.
%0 Journal Article
%1 nuhkat2021rapid
%A Nuhkat, M.
%A Brosche, M.
%A Stoelzle-Feix, S.
%A Dietrich, P.
%A Hedrich, R.
%A Roelfsema, M. R. G.
%A Kollist, H.
%D 2021
%J New Phytol
%K Calcium myOwn
%N 4
%P 1692-1702
%R 10.1111/nph.17711
%T Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells' ozone response
%U https://www.ncbi.nlm.nih.gov/pubmed/34482538
%V 232
%X Plant stress signalling involves bursts of reactive oxygen species (ROS), which can be mimicked by the application of acute pulses of ozone. Such ozone-pulses inhibit photosynthesis and trigger stomatal closure in a few minutes, but the signalling that underlies these responses remains largely unknown. We measured changes in Arabidopsis thaliana gas exchange after treatment with acute pulses of ozone and set up a system for simultaneous measurement of membrane potential and cytosolic calcium with the fluorescent reporter R-GECO1. We show that within 1 min, prior to stomatal closure, O(3) triggered a drop in whole-plant CO(2) uptake. Within this early phase, O(3) pulses (200-1000 ppb) elicited simultaneous membrane depolarization and cytosolic calcium increase, whereas these pulses had no long-term effect on either stomatal conductance or photosynthesis. In contrast, pulses of 5000 ppb O(3) induced cell death, systemic Ca(2+) signals and an irreversible drop in stomatal conductance and photosynthetic capacity. We conclude that mesophyll cells respond to ozone in a few seconds by distinct pattern of plasma membrane depolarizations accompanied by an increase in the cytosolic calcium ion (Ca(2+) ) level. These responses became systemic only at very high ozone concentrations. Thus, plants have rapid mechanism to sense and discriminate the strength of ozone signals.
@article{nuhkat2021rapid,
abstract = {Plant stress signalling involves bursts of reactive oxygen species (ROS), which can be mimicked by the application of acute pulses of ozone. Such ozone-pulses inhibit photosynthesis and trigger stomatal closure in a few minutes, but the signalling that underlies these responses remains largely unknown. We measured changes in Arabidopsis thaliana gas exchange after treatment with acute pulses of ozone and set up a system for simultaneous measurement of membrane potential and cytosolic calcium with the fluorescent reporter R-GECO1. We show that within 1 min, prior to stomatal closure, O(3) triggered a drop in whole-plant CO(2) uptake. Within this early phase, O(3) pulses (200-1000 ppb) elicited simultaneous membrane depolarization and cytosolic calcium increase, whereas these pulses had no long-term effect on either stomatal conductance or photosynthesis. In contrast, pulses of 5000 ppb O(3) induced cell death, systemic Ca(2+) signals and an irreversible drop in stomatal conductance and photosynthetic capacity. We conclude that mesophyll cells respond to ozone in a few seconds by distinct pattern of plasma membrane depolarizations accompanied by an increase in the cytosolic calcium ion (Ca(2+) ) level. These responses became systemic only at very high ozone concentrations. Thus, plants have rapid mechanism to sense and discriminate the strength of ozone signals.},
added-at = {2024-02-15T15:08:22.000+0100},
author = {Nuhkat, M. and Brosche, M. and Stoelzle-Feix, S. and Dietrich, P. and Hedrich, R. and Roelfsema, M. R. G. and Kollist, H.},
biburl = {https://www.bibsonomy.org/bibtex/22f380be5d2ad171e71a3b6df27635afa/jvsi_all},
doi = {10.1111/nph.17711},
interhash = {7385014d324b4c2b17d7d1cf1dc175c7},
intrahash = {2f380be5d2ad171e71a3b6df27635afa},
issn = {1469-8137 (Electronic)
0028-646X (Linking)},
journal = {New Phytol},
keywords = {Calcium myOwn},
note = {Nuhkat, Maris
Brosche, Mikael
Stoelzle-Feix, Sonja
Dietrich, Petra
Hedrich, Rainer
Roelfsema, M Rob G
Kollist, Hannes
eng
Research Support, Non-U.S. Gov't
England
2021/09/06
New Phytol. 2021 Nov;232(4):1692-1702. doi: 10.1111/nph.17711. Epub 2021 Sep 23.},
number = 4,
pages = {1692-1702},
timestamp = {2024-02-15T15:08:22.000+0100},
title = {Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells' ozone response},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/34482538},
volume = 232,
year = 2021
}