Whereas the role of calcium ions (Ca2+) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined.
Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca2+- and pH-dynamics revealed interconnections previously not described.
In tobacco PTs, we demonstrated Ca2+-dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca2+. In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca2+-increases and cytosolic acidification by c. 2 min, suggesting a Ca2+/pH-independent early pathogen signaling step. Imaging Ca2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca2+- and pH-responses.
We propose close interrelation in Ca2+- and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.
%0 Journal Article
%1 RN977
%A Li, K. K.
%A Prada, J.
%A Damineli, D. S. C.
%A Liese, A.
%A Romeis, T.
%A Dandekar, T.
%A Feijó, J. A.
%A Hedrich, R.
%A Konrad, K. R.
%D 2021
%J New Phytologist
%K (aba) abscisic acid myOwn
%N 6
%P 2292-2310
%R 10.1111/nph.17202
%T An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca
and H
reveals new insights into ion signaling in plants
%U /brokenurl#<Go to ISI>://WOS:000619171500001
%V 230
%X Whereas the role of calcium ions (Ca2+) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined.
Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca2+- and pH-dynamics revealed interconnections previously not described.
In tobacco PTs, we demonstrated Ca2+-dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca2+. In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca2+-increases and cytosolic acidification by c. 2 min, suggesting a Ca2+/pH-independent early pathogen signaling step. Imaging Ca2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca2+- and pH-responses.
We propose close interrelation in Ca2+- and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.
@article{RN977,
abstract = {Whereas the role of calcium ions (Ca2+) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined.
Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca2+- and pH-dynamics revealed interconnections previously not described.
In tobacco PTs, we demonstrated Ca2+-dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca2+. In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca2+-increases and cytosolic acidification by c. 2 min, suggesting a Ca2+/pH-independent early pathogen signaling step. Imaging Ca2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca2+- and pH-responses.
We propose close interrelation in Ca2+- and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Li, K. K. and Prada, J. and Damineli, D. S. C. and Liese, A. and Romeis, T. and Dandekar, T. and Feijó, J. A. and Hedrich, R. and Konrad, K. R.},
biburl = {https://www.bibsonomy.org/bibtex/241a6b9dd5905b31cde7845eab5e23e8b/rainerhedrich_2},
doi = {10.1111/nph.17202},
interhash = {2eebbaefd97f620ea1c42d7d0ce4902f},
intrahash = {41a6b9dd5905b31cde7845eab5e23e8b},
issn = {0028-646x},
journal = {New Phytologist},
keywords = {(aba) abscisic acid myOwn},
note = {Sd1fk
Times Cited:34
Cited References Count:147},
number = 6,
pages = {2292-2310},
timestamp = {2024-02-14T14:38:32.000+0100},
title = {An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca
and H
reveals new insights into ion signaling in plants},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000619171500001},
volume = 230,
year = 2021
}