Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.
%0 Journal Article
%1 Huisken:2009:Development:19465594
%A Huisken, J
%A Stainier, D Y
%D 2009
%J Development
%K biology cells development embryos microscopy
%N 12
%P 1963-1975
%R 10.1242/dev.022426
%T Selective plane illumination microscopy techniques in developmental biology
%U http://www.ncbi.nlm.nih.gov/pubmed/19465594
%V 136
%X Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.
@article{Huisken:2009:Development:19465594,
abstract = {Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.},
added-at = {2014-09-09T18:13:36.000+0200},
author = {Huisken, J and Stainier, D Y},
biburl = {https://www.bibsonomy.org/bibtex/24ad3c02932185c987060198eb52f3219/alex_ruff},
description = {Selective plane illumination microscopy techniqu... [Development. 2009] - PubMed - NCBI},
doi = {10.1242/dev.022426},
interhash = {9f1d9fcfb02f64404f24ccf011e05ae3},
intrahash = {4ad3c02932185c987060198eb52f3219},
journal = {Development},
keywords = {biology cells development embryos microscopy},
month = jun,
number = 12,
pages = {1963-1975},
pmid = {19465594},
timestamp = {2014-09-09T18:13:36.000+0200},
title = {Selective plane illumination microscopy techniques in developmental biology},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19465594},
volume = 136,
year = 2009
}