The recent explosion in the diversity of available fluorescent proteins (FPs) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPs are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPs provides a useful guide in narrowing down the options.
Description
A guide to choosing fluorescent proteins. [Nat Methods. 2005] - PubMed Result
%0 Journal Article
%1 Shaner:2005:Nat-Methods:16299475
%A Shaner, N C
%A Steinbach, P A
%A Tsien, R Y
%D 2005
%J Nat Methods
%K FP
%N 12
%P 905-909
%R 10.1038/nmeth819
%T A guide to choosing fluorescent proteins
%U http://www.ncbi.nlm.nih.gov/pubmed/16299475?dopt=Abstract
%V 2
%X The recent explosion in the diversity of available fluorescent proteins (FPs) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPs are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPs provides a useful guide in narrowing down the options.
@article{Shaner:2005:Nat-Methods:16299475,
abstract = {The recent explosion in the diversity of available fluorescent proteins (FPs) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPs are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these questions, a unified characterization of the best available FPs provides a useful guide in narrowing down the options.},
added-at = {2008-12-13T17:49:43.000+0100},
author = {Shaner, N C and Steinbach, P A and Tsien, R Y},
biburl = {https://www.bibsonomy.org/bibtex/250e6cdae05322e8f119983e62e48dad0/ben8bibsonomy},
description = {A guide to choosing fluorescent proteins. [Nat Methods. 2005] - PubMed Result},
doi = {10.1038/nmeth819},
interhash = {0c0407c8f56d99a9a8a5507c0501ecc1},
intrahash = {50e6cdae05322e8f119983e62e48dad0},
journal = {Nat Methods},
keywords = {FP},
month = Dec,
number = 12,
pages = {905-909},
pmid = {16299475},
timestamp = {2008-12-13T17:49:43.000+0100},
title = {A guide to choosing fluorescent proteins},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16299475?dopt=Abstract},
volume = 2,
year = 2005
}