Fluorescence and UV/visible absorption properties were studied on
electron carrier proteins cytochromes c-553 and c3 and their Fe-free
forms (Fe-free cytochromes) over wide pH and temperature ranges
in comparison with cytochrome c, hemin and microperoxidase-9 (MP-9).
Although cytochromes c-553 and c3 were extracted from the same sulfate-reducing
bacterium, their absorption spectra at acidic pH and pH dependence
of fluorescence spectra were different. Cytochrome c-553 showed
photophysical characteristics similar to cytochrome c. This is attributed
to the influence of the ligands of the fifth and sixth positions
of the heme iron in cytochromes c-553 and c3, which was confirmed
by the experiments on MP-9. Through the temperature-dependent fluorescence
spectrum and time profile, the coexistence of protonated and deprotonated
forms of Fe-free cytochromes at neutral pH was observed. The fluorescence
in native cytochromes was observed at acidic pH, whereas MP-9 and
hemin showed no fluorescence at the same condition.
%0 Journal Article
%1 citeulike:895360
%A Abe, Miriam
%A Yagi, Tatsuhiko
%A Itagaki, Hideyuki
%A Nagamura, Toshihiko
%D 1999
%J Journal of Photochemistry and Photobiology A: Chemistry
%K fluorescence heme-protein heme iron uv p450 reference
%N 2
%P 125--133
%R 10.1016/S1010-6030(98)00420-1
%T Photophysical properties of cytochromes c-553 and c3 extracted from
Desulfovibrio vulgaris Miyazaki
%U http://dx.doi.org/10.1016/S1010-6030(98)00420-1
%V 120
%X Fluorescence and UV/visible absorption properties were studied on
electron carrier proteins cytochromes c-553 and c3 and their Fe-free
forms (Fe-free cytochromes) over wide pH and temperature ranges
in comparison with cytochrome c, hemin and microperoxidase-9 (MP-9).
Although cytochromes c-553 and c3 were extracted from the same sulfate-reducing
bacterium, their absorption spectra at acidic pH and pH dependence
of fluorescence spectra were different. Cytochrome c-553 showed
photophysical characteristics similar to cytochrome c. This is attributed
to the influence of the ligands of the fifth and sixth positions
of the heme iron in cytochromes c-553 and c3, which was confirmed
by the experiments on MP-9. Through the temperature-dependent fluorescence
spectrum and time profile, the coexistence of protonated and deprotonated
forms of Fe-free cytochromes at neutral pH was observed. The fluorescence
in native cytochromes was observed at acidic pH, whereas MP-9 and
hemin showed no fluorescence at the same condition.
@article{citeulike:895360,
abstract = {Fluorescence and UV/visible absorption properties were studied on
electron carrier proteins cytochromes c-553 and c3 and their Fe-free
forms (Fe-free cytochromes) over wide pH and temperature ranges
in comparison with cytochrome c, hemin and microperoxidase-9 (MP-9).
Although cytochromes c-553 and c3 were extracted from the same sulfate-reducing
bacterium, their absorption spectra at acidic pH and pH dependence
of fluorescence spectra were different. Cytochrome c-553 showed
photophysical characteristics similar to cytochrome c. This is attributed
to the influence of the ligands of the fifth and sixth positions
of the heme iron in cytochromes c-553 and c3, which was confirmed
by the experiments on MP-9. Through the temperature-dependent fluorescence
spectrum and time profile, the coexistence of protonated and deprotonated
forms of Fe-free cytochromes at neutral pH was observed. The fluorescence
in native cytochromes was observed at acidic pH, whereas MP-9 and
hemin showed no fluorescence at the same condition.},
added-at = {2007-02-02T11:54:15.000+0100},
author = {Abe, Miriam and Yagi, Tatsuhiko and Itagaki, Hideyuki and Nagamura, Toshihiko},
biburl = {https://www.bibsonomy.org/bibtex/262c8f0cb62662c8eda5abd79f0a5ff15/robert},
citeulike-article-id = {895360},
doi = {10.1016/S1010-6030(98)00420-1},
interhash = {faeb8c631b7d8bce97abcda95c65c55b},
intrahash = {62c8f0cb62662c8eda5abd79f0a5ff15},
journal = {Journal of Photochemistry and Photobiology A: Chemistry},
keywords = {fluorescence heme-protein heme iron uv p450 reference},
month = {January},
number = 2,
pages = {125--133},
priority = {2},
timestamp = {2007-02-02T11:54:15.000+0100},
title = {Photophysical properties of cytochromes c-553 and c3 extracted from
\textit{Desulfovibrio vulgaris Miyazaki}},
url = {http://dx.doi.org/10.1016/S1010-6030(98)00420-1},
volume = 120,
year = 1999
}