The current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300 mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09 ng ml-1 IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30 min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood.
%0 Journal Article
%1 citeulike:671176
%A Kreuzer, M. P.
%A O’sullivan, C. K.
%A Pravda, M.
%A Guilbault, G. G.
%D 2001
%J Analytica Chimica Acta
%K a-p immunoassay immunosensor electrochemistry elisa
%N 1
%P 45--53
%R 10.1016/S0003-2670(01)01137-0
%T Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples
%U http://www.sciencedirect.com/science/article/B6TF4-43RV9KY-5/2/6b28a768a1b945c85d37399e983c0d64
%V 442
%X The current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300 mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09 ng ml-1 IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30 min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood.
@article{citeulike:671176,
abstract = {The current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300 mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09 ng ml-1 IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30 min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood.},
added-at = {2006-07-07T01:10:50.000+0200},
author = {Kreuzer, M. P. and O\’sullivan, C. K. and Pravda, M. and Guilbault, G. G.},
biburl = {https://www.bibsonomy.org/bibtex/2778792ee2e274faea22e6321947b494b/biblio24},
citeulike-article-id = {671176},
doi = {10.1016/S0003-2670(01)01137-0},
interhash = {0c1800a6f619d5b6162be7a5a7531f99},
intrahash = {778792ee2e274faea22e6321947b494b},
journal = {Analytica Chimica Acta},
keywords = {a-p immunoassay immunosensor electrochemistry elisa},
month = {August},
number = 1,
pages = {45--53},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples},
url = {http://www.sciencedirect.com/science/article/B6TF4-43RV9KY-5/2/6b28a768a1b945c85d37399e983c0d64},
volume = 442,
year = 2001
}