In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate (cAMP) guide cell aggregation and commitment to form fruiting bodies. It is unclear whether these oscillations are an intrinsic property of individual cells or if they exist only as a population-level phenomenon. Here, we showed by live-cell imaging of intact cell populations that pulses originate from a discrete location despite constant exchange of cells to and from the region. In a perfusion chamber, both isolated single cells and cell populations switched from quiescence to rhythmic activity depending on the concentration of extracellular cAMP. A quantitative analysis showed that stochastic pulsing of individual cells below the threshold concentration of extracellular cAMP plays a critical role in the onset of collective behavior.
%0 Journal Article
%1 Gregor2010Onset
%A Gregor, Thomas
%A Fujimoto, Koichi
%A Masaki, Noritaka
%A Sawai, Satoshi
%D 2010
%I American Association for the Advancement of Science
%J Science (New York, N.Y.)
%K communities complex-systems emergence groupthink social
%N 5981
%P 1021--1025
%R 10.1126/science.1183415
%T The onset of collective behavior in social amoebae.
%U http://dx.doi.org/10.1126/science.1183415
%V 328
%X In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate (cAMP) guide cell aggregation and commitment to form fruiting bodies. It is unclear whether these oscillations are an intrinsic property of individual cells or if they exist only as a population-level phenomenon. Here, we showed by live-cell imaging of intact cell populations that pulses originate from a discrete location despite constant exchange of cells to and from the region. In a perfusion chamber, both isolated single cells and cell populations switched from quiescence to rhythmic activity depending on the concentration of extracellular cAMP. A quantitative analysis showed that stochastic pulsing of individual cells below the threshold concentration of extracellular cAMP plays a critical role in the onset of collective behavior.
@article{Gregor2010Onset,
abstract = {In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate ({cAMP}) guide cell aggregation and commitment to form fruiting bodies. It is unclear whether these oscillations are an intrinsic property of individual cells or if they exist only as a population-level phenomenon. Here, we showed by live-cell imaging of intact cell populations that pulses originate from a discrete location despite constant exchange of cells to and from the region. In a perfusion chamber, both isolated single cells and cell populations switched from quiescence to rhythmic activity depending on the concentration of extracellular {cAMP}. A quantitative analysis showed that stochastic pulsing of individual cells below the threshold concentration of extracellular {cAMP} plays a critical role in the onset of collective behavior.},
added-at = {2018-12-02T16:09:07.000+0100},
author = {Gregor, Thomas and Fujimoto, Koichi and Masaki, Noritaka and Sawai, Satoshi},
biburl = {https://www.bibsonomy.org/bibtex/27ad386f2eaee6d60ea7097373fcb9914/karthikraman},
citeulike-article-id = {7203881},
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citeulike-linkout-2 = {http://www.sciencemag.org/content/328/5981/1021.full.pdf},
citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/328/5981/1021},
citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/20413456},
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day = 21,
doi = {10.1126/science.1183415},
interhash = {e86484c7edbf583b09df9cdc0e4e3d49},
intrahash = {7ad386f2eaee6d60ea7097373fcb9914},
issn = {1095-9203},
journal = {Science (New York, N.Y.)},
keywords = {communities complex-systems emergence groupthink social},
month = may,
number = 5981,
pages = {1021--1025},
pmid = {20413456},
posted-at = {2010-05-21 09:01:33},
priority = {2},
publisher = {American Association for the Advancement of Science},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {The onset of collective behavior in social amoebae.},
url = {http://dx.doi.org/10.1126/science.1183415},
volume = 328,
year = 2010
}