Vascular endothelial growth factor receptor 3 (VEGFR-3) is a major inducer of lymphangiogenic signalling and seems to be involved also in angiogenesis. Since both processes are closely linked with tumor metastasis this study investigated the expression of VEGFR-3 in tumor-associated vessels in colorectal carcinomas and evaluated its relevance for lymphogenous and hematogenous metastasis. In a comparative study between microvascular endothelial cells isolated from the tumor (HCTEC) and the corresponding non-neoplastic tissue (HCMEC) from five patients with colorectal cancer VEGFR-3 expression was measured using a specific ELISA. The expression pattern was individually different, with cases showing reduced, elevated and unchanged protein levels. Under hypoxic culture conditions (3% O2 for 24 h), which are more realistic for the tumor situation, the levels remained unchanged. In contrast, hypoxia exposure of macrovascular human umbilical vein endothelial cells (HUVEC) led to a consistent
%0 Journal Article
%1 Jayasinghe.2009
%A Jayasinghe, C.
%A Simiantonaki, N.
%A Michel-Schmidt, R.
%A Kirkpatrick, C. J.
%D 2009
%J Oncol.Rep.
%K Antibody Assay Blood Carcinoma Cell Cells Colon Colorectal Cytometry Endothelial Endothelium Enzyme-Linked Factor Flow Fluorescent Growth Human Humans Hypoxia Immunoenzyme Immunohistochemistry Immunosorbent Lymphatic Metastasis Neoplasm Neoplasms Neovascularization Pathologic Prognosis Receptor-3 Technique Techniques Vascular Vessels blood cells metabolism pathology protein supply
%N 5
%P 1093-1100
%T Endothelial VEGFR-3 expression in colorectal carcinomas is associated with hematogenous metastasis
%U PM:19787226
%V 22
%X Vascular endothelial growth factor receptor 3 (VEGFR-3) is a major inducer of lymphangiogenic signalling and seems to be involved also in angiogenesis. Since both processes are closely linked with tumor metastasis this study investigated the expression of VEGFR-3 in tumor-associated vessels in colorectal carcinomas and evaluated its relevance for lymphogenous and hematogenous metastasis. In a comparative study between microvascular endothelial cells isolated from the tumor (HCTEC) and the corresponding non-neoplastic tissue (HCMEC) from five patients with colorectal cancer VEGFR-3 expression was measured using a specific ELISA. The expression pattern was individually different, with cases showing reduced, elevated and unchanged protein levels. Under hypoxic culture conditions (3% O2 for 24 h), which are more realistic for the tumor situation, the levels remained unchanged. In contrast, hypoxia exposure of macrovascular human umbilical vein endothelial cells (HUVEC) led to a consistent
@article{Jayasinghe.2009,
abstract = {Vascular endothelial growth factor receptor 3 (VEGFR-3) is a major inducer of lymphangiogenic signalling and seems to be involved also in angiogenesis. Since both processes are closely linked with tumor metastasis this study investigated the expression of VEGFR-3 in tumor-associated vessels in colorectal carcinomas and evaluated its relevance for lymphogenous and hematogenous metastasis. In a comparative study between microvascular endothelial cells isolated from the tumor (HCTEC) and the corresponding non-neoplastic tissue (HCMEC) from five patients with colorectal cancer VEGFR-3 expression was measured using a specific ELISA. The expression pattern was individually different, with cases showing reduced, elevated and unchanged protein levels. Under hypoxic culture conditions (3% O2 for 24 h), which are more realistic for the tumor situation, the levels remained unchanged. In contrast, hypoxia exposure of macrovascular human umbilical vein endothelial cells (HUVEC) led to a consistent },
added-at = {2010-02-05T11:28:39.000+0100},
author = {Jayasinghe, C. and Simiantonaki, N. and Michel-Schmidt, R. and Kirkpatrick, C. J.},
biburl = {https://www.bibsonomy.org/bibtex/296ac6a63b45198cdccccde33a357efea/kanefendt},
interhash = {b49dcd540783916baed4357936c1b85f},
intrahash = {96ac6a63b45198cdccccde33a357efea},
journal = {Oncol.Rep.},
keywords = {Antibody Assay Blood Carcinoma Cell Cells Colon Colorectal Cytometry Endothelial Endothelium Enzyme-Linked Factor Flow Fluorescent Growth Human Humans Hypoxia Immunoenzyme Immunohistochemistry Immunosorbent Lymphatic Metastasis Neoplasm Neoplasms Neovascularization Pathologic Prognosis Receptor-3 Technique Techniques Vascular Vessels blood cells metabolism pathology protein supply},
number = 5,
pages = {1093-1100},
timestamp = {2010-02-05T11:28:51.000+0100},
title = {Endothelial VEGFR-3 expression in colorectal carcinomas is associated with hematogenous metastasis},
url = {PM:19787226},
volume = 22,
year = 2009
}