Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100\% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
%0 Journal Article
%1 aires-de-sousa_high_2006
%A Aires-de-Sousa, M
%A Boye, K
%A de Lencastre, H
%A Deplano, A
%A Enright, M C
%A Etienne, J
%A Friedrich, A
%A Harmsen, D
%A Holmes, A
%A Huijsdens, X W
%A Kearns, A M
%A Mellmann, A
%A Meugnier, H
%A Rasheed, J K
%A Spalburg, E
%A Strommenger, B
%A Struelens, M J
%A Tenover, F C
%A Thomas, J
%A Vogel, U
%A Westh, H
%A Xu, J
%A Witte, W
%D 2006
%J Journal of Clinical Microbiology
%K A, Analysis, Bacterial Bacterial, Humans, Infections, Laboratories, Observer Protein Reproducibility Results, Sequence Software, Staphylococcal Staphylococcus Techniques, Typing Variation, aureus of {DNA},
%N 2
%P 619--621
%R 10.1128/JCM.44.2.619-621.2006
%T High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study
%U http://www.ncbi.nlm.nih.gov/pubmed/16455927
%V 44
%X Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100\% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
@article{aires-de-sousa_high_2006,
abstract = {Current {DNA} amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, {DNA} sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100\% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus {DNA} samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.},
added-at = {2011-06-24T11:21:47.000+0200},
author = {{Aires-de-Sousa}, M and Boye, K and de Lencastre, H and Deplano, A and Enright, M C and Etienne, J and Friedrich, A and Harmsen, D and Holmes, A and Huijsdens, X W and Kearns, A M and Mellmann, A and Meugnier, H and Rasheed, J K and Spalburg, E and Strommenger, B and Struelens, M J and Tenover, F C and Thomas, J and Vogel, U and Westh, H and Xu, J and Witte, W},
biburl = {https://www.bibsonomy.org/bibtex/2b623829639fb8748aa7c57f022666b3a/ag_vogel},
doi = {10.1128/JCM.44.2.619-621.2006},
interhash = {900a4ec6dce0b62a57295564d99c68ea},
intrahash = {b623829639fb8748aa7c57f022666b3a},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
keywords = {A, Analysis, Bacterial Bacterial, Humans, Infections, Laboratories, Observer Protein Reproducibility Results, Sequence Software, Staphylococcal Staphylococcus Techniques, Typing Variation, aureus of {DNA},},
month = feb,
note = {{PMID:} 16455927},
number = 2,
pages = {619--621},
timestamp = {2011-06-24T11:21:54.000+0200},
title = {High interlaboratory reproducibility of {DNA} sequence-based typing of bacteria in a multicenter study},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16455927},
volume = 44,
year = 2006
}