New fluorescent ligands for adenosine receptors (ARs), obtained by
the insertion, in the N(6) position of NECA, of NBD-moieties with
linear alkyl spacers of increasing length, proved to possess a high
affinity and selectivity for the A(3) subtype expressed in CHO cells.
In fluorescence microscopy assays, compound 2d, the most active and
selective for human A(3)-AR, permitted visualization and localization
of this human receptor subtype, showing its potential suitability
for internalization and trafficking studies in living cells.
Macchia, M Salvetti, F Bertini, S Di Bussolo, V Gattuso, L Gesi,
M Hamdan, M Klotz, K N Laragione, T Lucacchini, A Minutolo, F Nencetti,
S Papi, C Tuscano, D Martini, C England Bioorganic & medicinal chemistry
letters Bioorg Med Chem Lett. 2001 Dec 3;11(23):3023-6.
%0 Journal Article
%1 Macchia2001
%A Macchia, M.
%A Salvetti, F.
%A Bertini, S.
%A Bussolo, V. Di
%A Gattuso, L.
%A Gesi, M.
%A Hamdan, M.
%A Klotz, K. N.
%A Laragione, T.
%A Lucacchini, A.
%A Minutolo, F.
%A Nencetti, S.
%A Papi, C.
%A Tuscano, D.
%A Martini, C.
%D 2001
%J Bioorg Med Chem Lett
%K A2A A3 Adenosine Adenosine-5'-(N-ethylcarboxamide)/*chemistry Animals Benzofurans/chemistry CHO Cell Compounds/chemistry Cricetinae Cultured Dyes/*chemistry/*metabolism Fluorescence Fluorescent Humans Ligands Membrane/metabolism Microscopy, Nitro P1/genetics/*metabolism Proteins/genetics/metabolism Purinergic Recombinant Receptor
%N 23
%P 3023-6
%T 7-Nitrobenzofurazan (NBD) derivatives of 5'-N-ethylcarboxamidoadenosine
(NECA) as new fluorescent probes for human A(3) adenosine receptors
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11714602
%V 11
%X New fluorescent ligands for adenosine receptors (ARs), obtained by
the insertion, in the N(6) position of NECA, of NBD-moieties with
linear alkyl spacers of increasing length, proved to possess a high
affinity and selectivity for the A(3) subtype expressed in CHO cells.
In fluorescence microscopy assays, compound 2d, the most active and
selective for human A(3)-AR, permitted visualization and localization
of this human receptor subtype, showing its potential suitability
for internalization and trafficking studies in living cells.
@article{Macchia2001,
abstract = {New fluorescent ligands for adenosine receptors (ARs), obtained by
the insertion, in the N(6) position of NECA, of NBD-moieties with
linear alkyl spacers of increasing length, proved to possess a high
affinity and selectivity for the A(3) subtype expressed in CHO cells.
In fluorescence microscopy assays, compound 2d, the most active and
selective for human A(3)-AR, permitted visualization and localization
of this human receptor subtype, showing its potential suitability
for internalization and trafficking studies in living cells.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Macchia, M. and Salvetti, F. and Bertini, S. and Bussolo, V. Di and Gattuso, L. and Gesi, M. and Hamdan, M. and Klotz, K. N. and Laragione, T. and Lucacchini, A. and Minutolo, F. and Nencetti, S. and Papi, C. and Tuscano, D. and Martini, C.},
biburl = {https://www.bibsonomy.org/bibtex/2baa09e72f79fd0252d92a4cc04ac77e7/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {9606c0528ff0013ba002c7355a2c7d80},
intrahash = {baa09e72f79fd0252d92a4cc04ac77e7},
issn = {0960-894X (Print) 0960-894X (Linking)},
journal = {Bioorg Med Chem Lett},
keywords = {A2A A3 Adenosine Adenosine-5'-(N-ethylcarboxamide)/*chemistry Animals Benzofurans/chemistry CHO Cell Compounds/chemistry Cricetinae Cultured Dyes/*chemistry/*metabolism Fluorescence Fluorescent Humans Ligands Membrane/metabolism Microscopy, Nitro P1/genetics/*metabolism Proteins/genetics/metabolism Purinergic Recombinant Receptor},
month = {Dec 3},
note = {Macchia, M Salvetti, F Bertini, S Di Bussolo, V Gattuso, L Gesi,
M Hamdan, M Klotz, K N Laragione, T Lucacchini, A Minutolo, F Nencetti,
S Papi, C Tuscano, D Martini, C England Bioorganic \& medicinal chemistry
letters Bioorg Med Chem Lett. 2001 Dec 3;11(23):3023-6.},
number = 23,
pages = {3023-6},
shorttitle = {7-Nitrobenzofurazan (NBD) derivatives of 5'-N-ethylcarboxamidoadenosine
(NECA) as new fluorescent probes for human A(3) adenosine receptors},
timestamp = {2010-12-14T18:20:50.000+0100},
title = {7-Nitrobenzofurazan (NBD) derivatives of 5'-N-ethylcarboxamidoadenosine
(NECA) as new fluorescent probes for human A(3) adenosine receptors},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11714602},
volume = 11,
year = 2001
}