A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.
%0 Journal Article
%1 valenza_first_2010
%A Valenza, Giuseppe
%A Joseph, Biju
%A Elias, Johannes
%A Claus, Heike
%A Oesterlein, Anett
%A Engelhardt, Kathrin
%A Turnwald, Doris
%A Frosch, Matthias
%A Abele-Horn, Marianne
%A Schoen, Christoph
%D 2010
%J Antimicrobial Agents and Chemotherapy
%K Agents, Bacterial, Ceftazidime, Drug Germany, Hospitals, Humans, Imipenem, Infections Microbial Pseudomonas Resistance, Sensitivity Tests, University, aeruginosa, ag_horn {Anti-Bacterial} {beta-Lactamases,}
%N 8
%P 3493--3497
%R 10.1128/AAC.00080-10
%T First survey of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a German university hospital
%U http://www.ncbi.nlm.nih.gov/pubmed/20498315
%V 54
%X A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.
@article{valenza_first_2010,
abstract = {A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase {(MBL)} production. Molecular analysis detected a {blaVIM-1} gene in the chromosome of one isolate and a {blaVIM-2} gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory {EDTA} combination disk test represents a valid alternative to the molecular investigation of {MBL} genes, making {MBL} detection possible in routine diagnostic laboratories.},
added-at = {2011-04-07T15:44:20.000+0200},
author = {Valenza, Giuseppe and Joseph, Biju and Elias, Johannes and Claus, Heike and Oesterlein, Anett and Engelhardt, Kathrin and Turnwald, Doris and Frosch, Matthias and {Abele-Horn}, Marianne and Schoen, Christoph},
biburl = {https://www.bibsonomy.org/bibtex/2c953b82d8158a1aebfe1619188eafc1e/hymi},
doi = {10.1128/AAC.00080-10},
interhash = {2159a4cbbbef0bcf34224e6cdbe362aa},
intrahash = {c953b82d8158a1aebfe1619188eafc1e},
issn = {1098-6596},
journal = {Antimicrobial Agents and Chemotherapy},
keywords = {Agents, Bacterial, Ceftazidime, Drug Germany, Hospitals, Humans, Imipenem, Infections Microbial Pseudomonas Resistance, Sensitivity Tests, University, aeruginosa, ag_horn {Anti-Bacterial} {beta-Lactamases,}},
month = aug,
note = {{PMID:} 20498315},
number = 8,
pages = {3493--3497},
timestamp = {2011-04-07T15:47:41.000+0200},
title = {First survey of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a German university hospital},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20498315},
volume = 54,
year = 2010
}