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First survey of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a German university hospital

, , , , , , , , , and . Antimicrobial Agents and Chemotherapy 54 (8): 3493--3497 (August 2010)PMID: 20498315.

Abstract

A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.

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DOI:
10.1128/AAC.00080-10
URL:
BibTeX key:
valenza_first_2010
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