%0 Journal Article %1 Christen2011Essential %A Christen, Beat %A Abeliuk, Eduardo %A Collier, John M. %A Kalogeraki, Virginia S. %A Passarelli, Ben %A Coller, John A. %A Fero, Michael J. %A McAdams, Harley H. %A Shapiro, Lucy %D 2011 %I Nature Publishing Group %J Molecular systems biology %K minimal-genome synthetic-biology %N 1 %R 10.1038/msb.2011.58 %T The essential genome of a bacterium. %U http://dx.doi.org/10.1038/msb.2011.58 %V 7 %X Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hyper-saturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 ORFs, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 RNA polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species.

@article{Christen2011Essential, abstract = { Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hyper-saturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 {ORFs}, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 {RNA} polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species. }, added-at = {2018-12-02T16:09:07.000+0100}, author = {Christen, Beat and Abeliuk, Eduardo and Collier, John M. and Kalogeraki, Virginia S. and Passarelli, Ben and Coller, John A. and Fero, Michael J. and McAdams, Harley H. and Shapiro, Lucy}, biburl = {https://www.bibsonomy.org/bibtex/2e49dfef46638d4c88c78910441f24467/karthikraman}, citeulike-article-id = {9729226}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/msb.2011.58}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/msb201158}, citeulike-linkout-2 = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3202797/}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/21878915}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=21878915}, day = 30, doi = {10.1038/msb.2011.58}, interhash = {1b61287d9af45a17e0f54a3c63974d74}, intrahash = {e49dfef46638d4c88c78910441f24467}, issn = {1744-4292}, journal = {Molecular systems biology}, keywords = {minimal-genome synthetic-biology}, month = aug, number = 1, pmcid = {PMC3202797}, pmid = {21878915}, posted-at = {2011-12-12 08:50:34}, priority = {2}, publisher = {Nature Publishing Group}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {The essential genome of a bacterium.}, url = {http://dx.doi.org/10.1038/msb.2011.58}, volume = 7, year = 2011 }