In the present study the role of Yersinia enterocolitica antigens in humoral immunity against secondary Y. enterocolitica infection has been investigated. For this purpose, BALB/c mice, 4 weeks after immunization by primary infection with a sublethal dose of various Y. enterocolitica strains and mutant strains, were challenged with a lethal dose of highly virulent Y. enterocolitica strains of serotype O:8. As evident from the survival rate and protection against bacterial growth in the spleens of mice, only immunization with wild-type or attenuated strains harbouring an intact virulence plasmid mediated protection against a lethal challenge. Protection by immunization with plasmid-harbouring strains correlated with persistence of the bacteria in spleens for at least 7 days after immunization and high serum titres of Yersinia-specific antibodies directed against both chromosomal and plasmid-encoded determinants. Furthermore, the adoptive transfer of the purified IgG fraction of a rabbit serum specific for the adhesin YadA encoded by virulence plasmid pO8 from Y. enterocolitica O:8 mediated protection against a lethal challenge with the serotype O:8 strain harbouring the virulence plasmid pO8 but did not mediate protection when this strain harboured the virulence plasmid pO9 from serotype O:9. In summary, the results demonstrate that in our experimental mouse infection model: (i) the presence of the intact virulence plasmid in an immunizing Y. enterocolitica strain is essential for induction of protection against a lethal challenge with highly virulent Y. enterocolitica and (ii) that antibodies against plasmid-encoded determinants of Y. enterocolitica, especially YadA, are of major importance for achievement of protective immunity in mice.
%0 Journal Article
%1 vogel_role_1993
%A Vogel, U
%A Autenrieth, I B
%A Berner, R
%A Heesemann, J
%D 1993
%J Microbial Pathogenesis
%K 50, Active, Animals, Antibodies, Antibody Antigens, Bacterial, C, Dose Female, Formation, G, Immunity, Immunization, Immunoglobulin Inbred Infections Lethal Mice, Passive, Specificity, Spleen, Virulence, Yersinia enterocolitica, {BALB}
%N 1
%P 23--36
%R 10.1006/mpat.1993.1054
%T Role of plasmid-encoded antigens of Yersinia enterocolitica in humoral immunity against secondary Y. enterocolitica infection in mice
%U http://www.ncbi.nlm.nih.gov/pubmed/8412625
%V 15
%X In the present study the role of Yersinia enterocolitica antigens in humoral immunity against secondary Y. enterocolitica infection has been investigated. For this purpose, BALB/c mice, 4 weeks after immunization by primary infection with a sublethal dose of various Y. enterocolitica strains and mutant strains, were challenged with a lethal dose of highly virulent Y. enterocolitica strains of serotype O:8. As evident from the survival rate and protection against bacterial growth in the spleens of mice, only immunization with wild-type or attenuated strains harbouring an intact virulence plasmid mediated protection against a lethal challenge. Protection by immunization with plasmid-harbouring strains correlated with persistence of the bacteria in spleens for at least 7 days after immunization and high serum titres of Yersinia-specific antibodies directed against both chromosomal and plasmid-encoded determinants. Furthermore, the adoptive transfer of the purified IgG fraction of a rabbit serum specific for the adhesin YadA encoded by virulence plasmid pO8 from Y. enterocolitica O:8 mediated protection against a lethal challenge with the serotype O:8 strain harbouring the virulence plasmid pO8 but did not mediate protection when this strain harboured the virulence plasmid pO9 from serotype O:9. In summary, the results demonstrate that in our experimental mouse infection model: (i) the presence of the intact virulence plasmid in an immunizing Y. enterocolitica strain is essential for induction of protection against a lethal challenge with highly virulent Y. enterocolitica and (ii) that antibodies against plasmid-encoded determinants of Y. enterocolitica, especially YadA, are of major importance for achievement of protective immunity in mice.
@article{vogel_role_1993,
abstract = {In the present study the role of Yersinia enterocolitica antigens in humoral immunity against secondary Y. enterocolitica infection has been investigated. For this purpose, {BALB/c} mice, 4 weeks after immunization by primary infection with a sublethal dose of various Y. enterocolitica strains and mutant strains, were challenged with a lethal dose of highly virulent Y. enterocolitica strains of serotype O:8. As evident from the survival rate and protection against bacterial growth in the spleens of mice, only immunization with wild-type or attenuated strains harbouring an intact virulence plasmid mediated protection against a lethal challenge. Protection by immunization with plasmid-harbouring strains correlated with persistence of the bacteria in spleens for at least 7 days after immunization and high serum titres of Yersinia-specific antibodies directed against both chromosomal and plasmid-encoded determinants. Furthermore, the adoptive transfer of the purified {IgG} fraction of a rabbit serum specific for the adhesin {YadA} encoded by virulence plasmid {pO8} from Y. enterocolitica O:8 mediated protection against a lethal challenge with the serotype O:8 strain harbouring the virulence plasmid {pO8} but did not mediate protection when this strain harboured the virulence plasmid {pO9} from serotype O:9. In summary, the results demonstrate that in our experimental mouse infection model: (i) the presence of the intact virulence plasmid in an immunizing Y. enterocolitica strain is essential for induction of protection against a lethal challenge with highly virulent Y. enterocolitica and (ii) that antibodies against plasmid-encoded determinants of Y. enterocolitica, especially {YadA}, are of major importance for achievement of protective immunity in mice.},
added-at = {2011-06-24T11:21:47.000+0200},
author = {Vogel, U and Autenrieth, I B and Berner, R and Heesemann, J},
biburl = {https://www.bibsonomy.org/bibtex/2fa0d667a991e1ad9d1b8a88033d07d88/ag_vogel},
doi = {10.1006/mpat.1993.1054},
interhash = {d5266a529cbb9fc8bc5f8da745335c70},
intrahash = {fa0d667a991e1ad9d1b8a88033d07d88},
issn = {0882-4010},
journal = {Microbial Pathogenesis},
keywords = {50, Active, Animals, Antibodies, Antibody Antigens, Bacterial, C, Dose Female, Formation, G, Immunity, Immunization, Immunoglobulin Inbred Infections Lethal Mice, Passive, Specificity, Spleen, Virulence, Yersinia enterocolitica, {BALB}},
month = jul,
note = {{PMID:} 8412625},
number = 1,
pages = {23--36},
timestamp = {2011-06-24T11:21:54.000+0200},
title = {Role of plasmid-encoded antigens of Yersinia enterocolitica in humoral immunity against secondary Y. enterocolitica infection in mice},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8412625},
volume = 15,
year = 1993
}