%0 Journal Article %1 McMaster.1995 %A McMaster, M. L. %A Gessler, M. %A Stanbridge, E. J. %A Weissman, B. E. %D 1995 %J Cell Growth Differ %K *Cell *Genes;Wilms 11 Animals Base Blotting;Northern Cell Chain Chromosomes;Human;Pair DNA DNA-Binding Data Division Factor Factors/*biosynthesis Female Growth Humans II/biosynthesis Insulin-Like Kidney Line Mice Mice;Nude Molecular Neoplasms/*genetics Polymerase Primers Proteins Proteins/*biosynthesis Proteins/biosynthesis Reaction Recombinant Sequence Transcription Transfection Transformation;Neoplastic Transplantation;Heterologous Tumor Tumor/*genetics WT1 Wilms %N 12 %P 1609--1617 %T WT1 expression alters tumorigenicity of the G401 kidney-derived cell line %V 6 %X Recent studies have implicated a loss of WT1 tumor suppressor gene function in the development of Wilms' tumor (WT). To determine the potential biological consequences of WT1 inactivation in these tumors, we transfected two different splice variant forms of this gene into the pediatric kidney-derived cell line G401. Introduction of this gene caused no detectable effects on the population doubling times of the cell line; proliferative capacity in soft agar was not significantly affected. However, the expression of this gene altered the morphology of the cells in culture and caused a significant suppression of tumorigenicity in the cells. Thus, the expression of WT1 in a pediatric kidney-derived cell line lacking endogenous WT1 production caused demonstrable effects on its in vitro and in vivo growth properties. These data strengthen the concept for a central role for WT1 inactivation in the etiology of this disease.

@article{McMaster.1995, abstract = {Recent studies have implicated a loss of WT1 tumor suppressor gene function in the development of Wilms' tumor (WT). To determine the potential biological consequences of WT1 inactivation in these tumors, we transfected two different splice variant forms of this gene into the pediatric kidney-derived cell line G401. Introduction of this gene caused no detectable effects on the population doubling times of the cell line; proliferative capacity in soft agar was not significantly affected. However, the expression of this gene altered the morphology of the cells in culture and caused a significant suppression of tumorigenicity in the cells. Thus, the expression of WT1 in a pediatric kidney-derived cell line lacking endogenous WT1 production caused demonstrable effects on its in vitro and in vivo growth properties. These data strengthen the concept for a central role for WT1 inactivation in the etiology of this disease.}, added-at = {2013-01-29T13:47:26.000+0100}, author = {McMaster, M. L. and Gessler, M. and Stanbridge, E. J. and Weissman, B. E.}, biburl = {https://www.bibsonomy.org/bibtex/25f1a4798cd5271a24cae024163065aa6/ebch}, interhash = {592963d1f8161da56296ecb0f16d835b}, intrahash = {5f1a4798cd5271a24cae024163065aa6}, journal = {Cell Growth Differ}, keywords = {*Cell *Genes;Wilms 11 Animals Base Blotting;Northern Cell Chain Chromosomes;Human;Pair DNA DNA-Binding Data Division Factor Factors/*biosynthesis Female Growth Humans II/biosynthesis Insulin-Like Kidney Line Mice Mice;Nude Molecular Neoplasms/*genetics Polymerase Primers Proteins Proteins/*biosynthesis Proteins/biosynthesis Reaction Recombinant Sequence Transcription Transfection Transformation;Neoplastic Transplantation;Heterologous Tumor Tumor/*genetics WT1 Wilms}, number = 12, pages = {1609--1617}, timestamp = {2013-01-29T13:47:29.000+0100}, title = {WT1 expression alters tumorigenicity of the G401 kidney-derived cell line}, volume = 6, year = 1995 }