%0 Journal Article %1 Freund1994 %A Freund, S. %A Ungerer, M. %A Lohse, M. J. %D 1994 %J Naunyn Schmiedebergs Arch Pharmacol %K & Adenylate Animals Binding, Bordetella/pharmacology Brain/drug C CHO Cell Competitive Cricetinae Cricetulus Cyclase Cyclase/*metabolism Factors, Inositol Membrane/metabolism P1/agonists/antagonists Pertussis Phosphates/metabolism Phospholipases/*metabolism Proteins/metabolism Purinergic Rats Recombinant Signal Toxin Transduction Type Virulence Xanthines/metabolism/pharmacology effects/metabolism inhibitors/*metabolism Receptor %N 1 %P 49-56 %T A1 adenosine receptors expressed in CHO-cells couple to adenylyl cyclase and to phospholipase C %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7935854 %V 350 %X A1 adenosine receptors are in general coupled to inhibition of adenylyl cyclase, but have more recently been reported to be capable of also activating phospholipase C. The present study was done in order to investigate whether these different effects can be elicited by a single A1 receptor, or whether A1 receptor subtypes have to be invoked. The cDNA of a rat brain A1 adenosine receptor was stably expressed in CHO-cells, resulting in clones with varying receptor densities; a clone expressing 1.9 pmol receptors/mg membrane protein was used for further characterization. The ligand binding properties of the expressed receptors were typical for the rat A1 adenosine receptor. A1 receptor agonists caused a concentration-dependent inhibition of adenylyl cyclase activity in the membranes, with maximal inhibition by 70%. A1 receptor stimulation also caused concentration-dependent stimulation of inositol phosphate generation in these cells, with maximal effects of 300%. Both adenylyl cyclase inhibition and enhancement of inositol phosphate generation were essentially abolished after pretreatment of the cells with pertussis toxin. These results indicate that a single A1 adenosine receptor can couple to two effector pathways, and that both effectors are activated via pertussis toxin sensitive G proteins.

@article{Freund1994, abstract = {A1 adenosine receptors are in general coupled to inhibition of adenylyl cyclase, but have more recently been reported to be capable of also activating phospholipase C. The present study was done in order to investigate whether these different effects can be elicited by a single A1 receptor, or whether A1 receptor subtypes have to be invoked. The cDNA of a rat brain A1 adenosine receptor was stably expressed in CHO-cells, resulting in clones with varying receptor densities; a clone expressing 1.9 pmol receptors/mg membrane protein was used for further characterization. The ligand binding properties of the expressed receptors were typical for the rat A1 adenosine receptor. A1 receptor agonists caused a concentration-dependent inhibition of adenylyl cyclase activity in the membranes, with maximal inhibition by 70%. A1 receptor stimulation also caused concentration-dependent stimulation of inositol phosphate generation in these cells, with maximal effects of 300%. Both adenylyl cyclase inhibition and enhancement of inositol phosphate generation were essentially abolished after pretreatment of the cells with pertussis toxin. These results indicate that a single A1 adenosine receptor can couple to two effector pathways, and that both effectors are activated via pertussis toxin sensitive G proteins.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Freund, S. and Ungerer, M. and Lohse, M. J.}, biburl = {https://www.bibsonomy.org/bibtex/2680a4dfe80e12e0224c195d5496795b6/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {9a7464806c0445f7c38f1e48fc2b6b25}, intrahash = {680a4dfe80e12e0224c195d5496795b6}, issn = {0028-1298 (Print) 0028-1298 (Linking)}, journal = {Naunyn Schmiedebergs Arch Pharmacol}, keywords = {& Adenylate Animals Binding, Bordetella/pharmacology Brain/drug C CHO Cell Competitive Cricetinae Cricetulus Cyclase Cyclase/*metabolism Factors, Inositol Membrane/metabolism P1/agonists/antagonists Pertussis Phosphates/metabolism Phospholipases/*metabolism Proteins/metabolism Purinergic Rats Recombinant Signal Toxin Transduction Type Virulence Xanthines/metabolism/pharmacology effects/metabolism inhibitors/*metabolism Receptor}, month = Jul, note = {Freund, S Ungerer, M Lohse, M J Research Support, Non-U.S. Gov't Germany Naunyn-Schmiedeberg's archives of pharmacology Naunyn Schmiedebergs Arch Pharmacol. 1994 Jul;350(1):49-56.}, number = 1, pages = {49-56}, shorttitle = {A1 adenosine receptors expressed in CHO-cells couple to adenylyl cyclase and to phospholipase C}, timestamp = {2010-12-14T18:20:38.000+0100}, title = {A1 adenosine receptors expressed in CHO-cells couple to adenylyl cyclase and to phospholipase C}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7935854}, volume = 350, year = 1994 }