%0 Journal Article %1 Marx_2001_1151 %A Marx, S. O. %A Gaburjakova, J. %A Gaburjakova, M. %A Henrikson, C. %A Ondrias, K. %A Marks, A. R. %D 2001 %J Circ. Res. %K (Alcohol 2, 4, ADP AMP-Dependent Activation, Adhesion, Agents, Angioplasty, Animals, Aorta, Arteriosclerosis, Barium, Bilayers, Botulinum C57BL, CDC2-CDC28 Caffeine, Calcineurin, Calcium-Binding Cardiac, Cardiomyopathy, Carrier Cell Cells, Centrifugation, Chains, Chambers, Channel Chloride, Clinical Coloring Contraction, Coronary Coronary, Count, Culture, Cultured, Cycle Cyclic Cyclin-Dependent Death, Density Diffusion Dilated, Division, Dogs, Dose-Response Down-Regulation, Drug, Enzyme Factor Fibroblast Gating, Gov't, Gradient, Graft Group Growth Heavy Heterozygote, Homozygote, Humans, Hyperplasia, Immunoblotting, Inbred Inhibitor Ion K, Kinase Kinases, Knockout, Lipid Macromolecular Magnesium Membrane Mice, Microsomes, Microtubule-Associated Movement, Muscle, Myocardial Myocardium, Myosin Non-U.S. Occlusion, P.H.S., Percutaneous Phosphorylation, Phosphotransferases Potentials, Protein Proteins, Relationship, Ribose Substances, Survival, Toxins, Transferases, Trials, p27, %N 11 %P 1151--1158 %T Coupled gating between cardiac calcium release channels (ryanodine receptors). %U http://circres.ahajournals.org/cgi/content/full/88/11/1151 %V 88 %X Excitation-contraction coupling in heart muscle requires the activation of Ca$^2+$-release channels/type 2 ryanodine receptors (RyR2s) by Ca$^2+$ influx. RyR2s are arranged on the sarcoplasmic reticular membrane in closely packed arrays such that their large cytoplasmic domains contact one another. We now show that multiple RyR2s can be isolated under conditions such that they remain physically coupled to one another. When these coupled channels are examined in planar lipid bilayers, multiple channels exhibit simultaneous gating, termed "coupled gating." Removal of the regulatory subunit, the FK506 binding protein (FKBP12.6), functionally but not physically uncouples multiple RyR2 channels. Coupled gating between RyR2 channels may be an important regulatory mechanism in excitation-contraction coupling as well as in other signaling pathways involving intracellular Ca$^2+$ release.

@article{Marx_2001_1151, abstract = {Excitation-contraction coupling in heart muscle requires the activation of {C}a$^{2+}$-release channels/type 2 ryanodine receptors (RyR2s) by {C}a$^{2+}$ influx. RyR2s are arranged on the sarcoplasmic reticular membrane in closely packed arrays such that their large cytoplasmic domains contact one another. We now show that multiple RyR2s can be isolated under conditions such that they remain physically coupled to one another. When these coupled channels are examined in planar lipid bilayers, multiple channels exhibit simultaneous gating, termed "coupled gating." Removal of the regulatory subunit, the FK506 binding protein ({FKBP}12.6), functionally but not physically uncouples multiple RyR2 channels. Coupled gating between RyR2 channels may be an important regulatory mechanism in excitation-contraction coupling as well as in other signaling pathways involving intracellular {C}a$^{2+}$ release.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Marx, S. O. and Gaburjakova, J. and Gaburjakova, M. and Henrikson, C. and Ondrias, K. and Marks, A. R.}, biburl = {https://www.bibsonomy.org/bibtex/2c6d1ca432c1c138fe2f695517b1d320d/hake}, description = {The whole bibliography file I use.}, file = {Marx_2001_1151.pdf:Marx_2001_1151.pdf:PDF}, interhash = {a06e8a5413bf070ef3f1bd135b9150c4}, intrahash = {c6d1ca432c1c138fe2f695517b1d320d}, journal = {Circ. Res.}, keywords = {(Alcohol 2, 4, ADP AMP-Dependent Activation, Adhesion, Agents, Angioplasty, Animals, Aorta, Arteriosclerosis, Barium, Bilayers, Botulinum C57BL, CDC2-CDC28 Caffeine, Calcineurin, Calcium-Binding Cardiac, Cardiomyopathy, Carrier Cell Cells, Centrifugation, Chains, Chambers, Channel Chloride, Clinical Coloring Contraction, Coronary Coronary, Count, Culture, Cultured, Cycle Cyclic Cyclin-Dependent Death, Density Diffusion Dilated, Division, Dogs, Dose-Response Down-Regulation, Drug, Enzyme Factor Fibroblast Gating, Gov't, Gradient, Graft Group Growth Heavy Heterozygote, Homozygote, Humans, Hyperplasia, Immunoblotting, Inbred Inhibitor Ion K, Kinase Kinases, Knockout, Lipid Macromolecular Magnesium Membrane Mice, Microsomes, Microtubule-Associated Movement, Muscle, Myocardial Myocardium, Myosin Non-U.S. Occlusion, P.H.S., Percutaneous Phosphorylation, Phosphotransferases Potentials, Protein Proteins, Relationship, Ribose Substances, Survival, Toxins, Transferases, Trials, p27,}, month = Jun, number = 11, pages = {1151--1158}, pmid = {11397781}, timestamp = {2009-06-03T11:21:22.000+0200}, title = {Coupled gating between cardiac calcium release channels (ryanodine receptors).}, url = {http://circres.ahajournals.org/cgi/content/full/88/11/1151}, volume = 88, year = 2001 }