%0 Journal Article %1 Zywietz2001 %A Zywietz, A. %A Gohla, A. %A Schmelz, M. %A Schultz, G. %A Offermanns, S. %D 2001 %J J Biol Chem %K *Bacterial Animals Bacterial Cultured GTP-Binding Gohla Gq-G11 Heterotrimeric Mice Pasteurella Phosphorylation Protein Proteins Proteins/metabolism/*physiology Subunits, Toxins/*pharmacology alpha multocida/*metabolism Cell %N 6 %P 3840-5 %T Pleiotropic effects of Pasteurella multocida toxin are mediated by Gq-dependent and -independent mechanisms. involvement of Gq but not G11 %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11062245 %V 276 %X Pasteurella multocida toxin (PMT) is a highly potent mitogen for a variety of cell types. PMT has been shown to induce various cellular signaling processes, and it has been suggested to function through the heterotrimeric G-proteins G(q)/G(11). To analyze the role of G(q)/G(11) in the action of PMT, we have studied the effect of the toxin in Galpha(q)/Galpha(11) double-deficient fibroblasts as well as in fibroblasts lacking only Galpha(q) or Galpha(11). Interestingly, formation of inositol phosphates in response to PMT was exclusively dependent on Galpha(q) but not on the closely related Galpha(11). Although Galpha(q)/Galpha(11) double-deficient and Galpha(q)-deficient cells did not respond with any production of inositol phosphates to PMT, PMT was still able to induce various other cellular effects in these cells, including the activation of Rho, the Rho-dependent formation of actin stress fibers and focal adhesions, as well as the stimulation of c-Jun N-terminal kinase and extracellular signal-regulated kinase. These data show that PMT leads to a variety of cellular effects that are mediated only in part by the heterotrimeric G-protein G(q).

@article{Zywietz2001, abstract = {Pasteurella multocida toxin (PMT) is a highly potent mitogen for a variety of cell types. PMT has been shown to induce various cellular signaling processes, and it has been suggested to function through the heterotrimeric G-proteins G(q)/G(11). To analyze the role of G(q)/G(11) in the action of PMT, we have studied the effect of the toxin in Galpha(q)/Galpha(11) double-deficient fibroblasts as well as in fibroblasts lacking only Galpha(q) or Galpha(11). Interestingly, formation of inositol phosphates in response to PMT was exclusively dependent on Galpha(q) but not on the closely related Galpha(11). Although Galpha(q)/Galpha(11) double-deficient and Galpha(q)-deficient cells did not respond with any production of inositol phosphates to PMT, PMT was still able to induce various other cellular effects in these cells, including the activation of Rho, the Rho-dependent formation of actin stress fibers and focal adhesions, as well as the stimulation of c-Jun N-terminal kinase and extracellular signal-regulated kinase. These data show that PMT leads to a variety of cellular effects that are mediated only in part by the heterotrimeric G-protein G(q).}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Zywietz, A. and Gohla, A. and Schmelz, M. and Schultz, G. and Offermanns, S.}, biburl = {https://www.bibsonomy.org/bibtex/2b660ae188f5b17ba6f065456a0afc040/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {b5f941d9e6baa067cdef48cce1493306}, intrahash = {b660ae188f5b17ba6f065456a0afc040}, issn = {0021-9258 (Print) 0021-9258 (Linking)}, journal = {J Biol Chem}, keywords = {*Bacterial Animals Bacterial Cultured GTP-Binding Gohla Gq-G11 Heterotrimeric Mice Pasteurella Phosphorylation Protein Proteins Proteins/metabolism/*physiology Subunits, Toxins/*pharmacology alpha multocida/*metabolism Cell}, month = {Feb 9}, note = {Zywietz, A Gohla, A Schmelz, M Schultz, G Offermanns, S Research Support, Non-U.S. Gov't United States The Journal of biological chemistry J Biol Chem. 2001 Feb 9;276(6):3840-5. Epub 2000 Nov 2.}, number = 6, pages = {3840-5}, shorttitle = {Pleiotropic effects of Pasteurella multocida toxin are mediated by Gq-dependent and -independent mechanisms. involvement of Gq but not G11}, timestamp = {2010-12-14T18:20:51.000+0100}, title = {Pleiotropic effects of Pasteurella multocida toxin are mediated by Gq-dependent and -independent mechanisms. involvement of Gq but not G11}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11062245}, volume = 276, year = 2001 }