Аннотация
We present a method for combining single axis tomography and serial
sectioning techniques to derive a three-dimensional reconstruction
of large structures at electron microscopic resolution. This serial-tomography
method allows the use of sufficiently thin sections to achieve adequate
resolution with electron tomography, yet enables the generation of
large reconstructions with considerably fewer sections than would
be required using a serial thin section reconstruction technique.
Serial thick sections (1-2 microns) are cut through the structure
of interest, tomographic volume reconstructions are obtained for
each section from a single axis tilt series, and the resulting series
of volumes are then aligned and combined to form a single large volume.
The serial-tomography method is illustrated with several samples,
including red blood cells, the Golgi apparatus, and a spiny dendrite
of a cortical pyramidal neuron. In some of these samples, the reconstruction
is compared to correlated light microscopic views. The resulting
large volume reconstructions appear to represent accurately the size
and shape of objects such as red blood cells and spiny dendrites.
The continuity of complex, tortuous structures such as the Golgi
apparatus is also maintained across serial volumes. These examples
demonstrate that it is possible to align and link a series of tomographic
volumes accurately and that serial-tomography is a useful method
for reconstructing relatively large structures without resorting
to large numbers of serial thin sections.
- 9343574
- animals,
- apparatus,
- catesbeiana,
- cell
- cells,
- computer
- computer-assisted,
- dendrites,
- electron,
- erythrocytes,
- ganglia,
- golgi
- image
- microscopy,
- neurons,
- processing,
- pyramidal
- rana
- rats,
- size,
- software,
- spinal,
- systems,
- tomography,
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