Abstract
A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was \textgreater99\% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/TTL64 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.
- ag_vogel
- analysis,
- and
- bacterial
- bacterial,
- bacterial_capsules
- bacteriological
- capsule,
- capsules,
- chain
- data,
- false
- genotype,
- haemophilus
- humans,
- imported
- infections,
- influenzae,
- insertional,
- je
- molecular
- molecular_epidemiology
- mutagenesis,
- negative
- polymerase
- primer,
- primers,
- reaction,
- reactions,
- sensitivity
- sequence
- serotype
- specificity,
- techniques,
- {dna}
- {dna},
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