%0 Journal Article %1 Nikolaev2006a %A Nikolaev, V. O. %A Gambaryan, S. %A Lohse, M. J. %D 2006 %J Nat Methods %K *Biosensing *Fluorescence AMP/analysis Cultured Cyclic Deletion Diester Energy GMP-Dependent GMP/*analysis Humans Hydrolases/*chemistry/genetics Kinases/*chemistry/genetics Phosphoric Protein Resonance Sequence Structure, Techniques Tertiary/genetics Transfer Cell %N 1 %P 23-5 %T Fluorescent sensors for rapid monitoring of intracellular cGMP %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16369548 %V 3 %X Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.

@article{Nikolaev2006a, abstract = {Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Nikolaev, V. O. and Gambaryan, S. and Lohse, M. J.}, biburl = {https://www.bibsonomy.org/bibtex/2ee04d9fed93ecdace33ccb4f3896389c/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {f32e9c1344d690a73655b6f7b9368920}, intrahash = {ee04d9fed93ecdace33ccb4f3896389c}, issn = {1548-7091 (Print) 1548-7091 (Linking)}, journal = {Nat Methods}, keywords = {*Biosensing *Fluorescence AMP/analysis Cultured Cyclic Deletion Diester Energy GMP-Dependent GMP/*analysis Humans Hydrolases/*chemistry/genetics Kinases/*chemistry/genetics Phosphoric Protein Resonance Sequence Structure, Techniques Tertiary/genetics Transfer Cell}, month = Jan, note = {Nikolaev, Viacheslav O Gambaryan, Stepan Lohse, Martin J Research Support, Non-U.S. Gov't United States Nature methods Nat Methods. 2006 Jan;3(1):23-5.}, number = 1, pages = {23-5}, shorttitle = {Fluorescent sensors for rapid monitoring of intracellular cGMP}, timestamp = {2010-12-14T18:20:50.000+0100}, title = {Fluorescent sensors for rapid monitoring of intracellular cGMP}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16369548}, volume = 3, year = 2006 }