%0 Journal Article %1 Chomczynski.1987 %A Chomczynski, P. %A Sacchi, N. %D 1987 %J Anal.Biochem. %K & Animal Animals Cell Chloroform Concentration Glands Guanidine Guanidines Humans Hydrogen-Ion Line Mammary Phenol Phenols RNA Rats Solutions analysis cells isolation purification %N 1 %P 156-159 %T Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction %U PM:2440339 %V 162 %X A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples

@article{Chomczynski.1987, abstract = {A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Chomczynski, P. and Sacchi, N.}, biburl = {https://www.bibsonomy.org/bibtex/2e0c24eb6bc035e5246ea5789b096d844/kanefendt}, interhash = {085d1c746c723e11d4872bae3265cada}, intrahash = {e0c24eb6bc035e5246ea5789b096d844}, journal = {Anal.Biochem.}, keywords = {& Animal Animals Cell Chloroform Concentration Glands Guanidine Guanidines Humans Hydrogen-Ion Line Mammary Phenol Phenols RNA Rats Solutions analysis cells isolation purification}, number = 1, pages = {156-159}, timestamp = {2010-02-05T11:28:44.000+0100}, title = {Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction}, url = {PM:2440339}, volume = 162, year = 1987 }