%0 Journal Article %1 Hjerde1999 %A Hjerde, T. %A Smidsrod, O. %A Christensen, B. E. %C 605 THIRD AVE, NEW YORK, NY 10158-0012 USA %D 1999 %I JOHN WILEY & SONS INC %J Biopolymers %K ; ;; [ISI:] acid-hydrolysis; carrageenan; chromotography; conformation; conformational depolymerization; exclusion helices kinetics; laser light low-angle molecular scattering; size transition; weight %N 1 %P 71 -- 80 %T Analysis of the conformational properties of kappa- and iota-carrageenan by size-exclusion chromatography combined with low-angle laser light scattering %V 49 %X The conformational properties of kappa-carrageenan in 0.2M LiI and iota-carrageenan in 0.2M LiCl were analyzed by size exclusion chromatography combined with low-angle laser light seat tering. Fractionated samples with narrow molecular weight distributions (M-w/M-n similar to 1.4) were used, and M-w in the disordered states were 35,000 (kappa-35) and 200,000 (kappa-200) for kappa-carrageenan and 65,000 (iota-65) and 170,000 (iota-170) for iota-carrageenan, respectively. The analyses were performed across a temperature range where the conformational transitions occurred, and at extremely low concentrations (2-50 mu g/mL) due to low amounts of sampler injected and the subsequent dilution occurring during the separation. The results indicate that a twofold increase of the molecular weight (M-w) occurs for kappa-carrageenan upon inducing the ordered conformation. For iota-carrageenan an additional increase in M-w, may take place, which is attributed to the strong tendency for aggregation of ordered chains especially at high molecular weights. The results thus suggest that both kappa- and iota-carrageenan are double (or multiple) stranded in their ordered conformations, within the concentration range studied here. (C) 1999 John Wiley & Sons, Inc.

@article{Hjerde1999, __markedentry = {[phpts:6]}, abstract = {The conformational properties of kappa-carrageenan in 0.2M LiI and iota-carrageenan in 0.2M LiCl were analyzed by size exclusion chromatography combined with low-angle laser light seat tering. Fractionated samples with narrow molecular weight distributions (M-w/M-n similar to 1.4) were used, and M-w in the disordered states were 35,000 (kappa-35) and 200,000 (kappa-200) for kappa-carrageenan and 65,000 (iota-65) and 170,000 (iota-170) for iota-carrageenan, respectively. The analyses were performed across a temperature range where the conformational transitions occurred, and at extremely low concentrations (2-50 mu g/mL) due to low amounts of sampler injected and the subsequent dilution occurring during the separation. The results indicate that a twofold increase of the molecular weight (M-w) occurs for kappa-carrageenan upon inducing the ordered conformation. For iota-carrageenan an additional increase in M-w, may take place, which is attributed to the strong tendency for aggregation of ordered chains especially at high molecular weights. The results thus suggest that both kappa- and iota-carrageenan are double (or multiple) stranded in their ordered conformations, within the concentration range studied here. (C) 1999 John Wiley & Sons, Inc.}, added-at = {2011-11-04T13:47:04.000+0100}, address = {605 THIRD AVE, NEW YORK, NY 10158-0012 USA}, author = {Hjerde, T. and Smidsrod, O. and Christensen, B. E.}, authoraddress = {Norwegian Univ Sci & Technol, Norwegian Biopolymer Lab, Dept Biotechnol, N-7034 Trondheim, Norway.}, biburl = {https://www.bibsonomy.org/bibtex/23a3d3606d08b512417ab40fb0ebeb821/pawelsikorski}, citedref = {ABEYSEKERA RM, 1993, CARBOHYD RES, V248, P225 ; ANDERSON NS, 1969, J MOL BIOL, V45, P85 ; AUSTEN KRJ, 1988, BIOPOLYMERS, V27, P139 ; CHRISTENSEN BE, 1993, MACROMOLECULES, V26, P6111 ; GRASDALEN H, 1981, MACROMOLECULES, V14, P1842 ; HJERDE T, 1994, CARBOHYD POLYM, V24, P265 ; HJERDE T, 1996, CARBOHYD RES, V288, P175 ; HJERDE T, 1998, MACROMOLECULES, V31, P1842 ; JONES RA, 1973, J CHEM SOC P2, P1608 ; LECACHEUX D, 1985, CARBOHYD POLYM, V5, P423 ; NERDAL W, 1993, J BIOMOL STRUCT DYN, V10, P785 ; NORTON IT, 1983, J CHEM SOC FARAD T 1, V79, P2475 ; NORTON IT, 1983, J CHEM SOC FARAD T 1, V79, P2501 ; PAOLETTI S, 1984, BIOPOLYMERS, V23, P1771 ; REES DA, 1969, ADV CARBOHYD CHEM BI, V24, P267 ; SLOOTMAEKERS D, 1988, INT J BIOL MACROMOL, V10, P160 ; SLOOTMAEKERS D, 1991, BIOPHYS CHEM, V41, P51 ; SMIDSROD O, 1980, CARBOHYD RES, V80, C11 ; SMIDSROD O, 1984, HYDROBIOLOGIA, V116, P178 ; SNOEREN THM, 1976, BIOCHIM BIOPHYS ACTA, V437, P264 ; STOKKE BT, 1993, INT J BIOL MACROMOL, V15, P63 ; VANDIJK JAP, 1987, J POLYM SCI POL LETT, V25, P145 ; VANNESTE K, 1994, MACROMOLECULES, V27, P7496 ; VIEBKE C, 1995, CARBOHYD POLYM, V27, P145 ; ZIMM BH, 1959, J CHEM PHYS, V31, P526}, interhash = {51420b6a0dc31b06aeabfcb4340841ad}, intrahash = {3a3d3606d08b512417ab40fb0ebeb821}, isifile-dt = {Article}, isifile-ga = {151QK}, isifile-j9 = {BIOPOLYMERS}, isifile-nr = {25}, isifile-pi = {NEW YORK}, isifile-rp = {Christensen, BE, Norwegian Univ Sci & Technol, Norwegian Biopolymer ; Lab, Dept Biotechnol, N-7034 Trondheim, Norway.}, isifile-sc = {Biochemistry & Molecular Biology; Biophysics}, isifile-tc = {15}, issn = {0006-3525}, journal = {Biopolymers}, keywords = {; ;; [ISI:] acid-hydrolysis; carrageenan; chromotography; conformation; conformational depolymerization; exclusion helices kinetics; laser light low-angle molecular scattering; size transition; weight}, language = {English}, month = JAN, number = 1, owner = {phpts}, pages = {71 -- 80}, publisher = {JOHN WILEY \& SONS INC}, size = {10 p.}, sourceid = {ISI:000077731800007}, timestamp = {2011-11-04T13:47:12.000+0100}, title = {Analysis of the conformational properties of kappa- and iota-carrageenan by size-exclusion chromatography combined with low-angle laser light scattering}, volume = 49, year = 1999 }