%0 Journal Article %1 Sano:2009:Plant-J:18778403 %A Sano, T %A Kutsuna, N %A Becker, D %A Hedrich, R %A Hasezawa, S %D 2009 %J Plant J %K myown %N 1 %P 55-64 %R 10.1111/j.1365-313X.2008.03672.x %T Outward-rectifying K+ channel activities regulate cell elongation and cell division of tobacco BY-2 cells %U https://www.ncbi.nlm.nih.gov/pubmed/18778403 %V 57 %X Potassium ions (K+) are required for plant growth and development, including cell division and cell elongation/expansion, which are mediated by the K+ transport system. In this study, we investigated the role of K+ in cell division using tobacco BY-2 protoplast cultures. Gene expression analysis revealed induction of the Shaker-like outward K+ channel gene, NTORK1, under cell-division conditions, whereas the inward K+ channel genes NKT1 and NtKC1 were induced under both cell-elongation and cell-division conditions. Repression of NTORK1 gene expression by expression of its antisense construct repressed cell division but accelerated cell elongation even under conditions promoting cell division. A decrease in the K+ content of cells and cellular osmotic pressure in dividing cells suggested that an increase in cell osmotic pressure by K+ uptake is not required for cell division. In contrast, K+ depletion, which reduced cell-division activity, decreased cytoplasmic pH as monitored using a fluorescent pH indicator, SNARF-1. Application of K+ or the cytoplasmic alkalizing reagent (NH(4))(2)SO(4) increased cytoplasmic pH and suppressed the reduction in cell-division activity. These results suggest that the K+ taken up into cells is used to regulate cytoplasmic pH during cell division.

@article{Sano:2009:Plant-J:18778403, abstract = {Potassium ions (K+) are required for plant growth and development, including cell division and cell elongation/expansion, which are mediated by the K+ transport system. In this study, we investigated the role of K+ in cell division using tobacco BY-2 protoplast cultures. Gene expression analysis revealed induction of the Shaker-like outward K+ channel gene, NTORK1, under cell-division conditions, whereas the inward K+ channel genes NKT1 and NtKC1 were induced under both cell-elongation and cell-division conditions. Repression of NTORK1 gene expression by expression of its antisense construct repressed cell division but accelerated cell elongation even under conditions promoting cell division. A decrease in the K+ content of cells and cellular osmotic pressure in dividing cells suggested that an increase in cell osmotic pressure by K+ uptake is not required for cell division. In contrast, K+ depletion, which reduced cell-division activity, decreased cytoplasmic pH as monitored using a fluorescent pH indicator, SNARF-1. Application of K+ or the cytoplasmic alkalizing reagent (NH(4))(2)SO(4) increased cytoplasmic pH and suppressed the reduction in cell-division activity. These results suggest that the K+ taken up into cells is used to regulate cytoplasmic pH during cell division.}, added-at = {2017-03-21T20:39:31.000+0100}, author = {Sano, T and Kutsuna, N and Becker, D and Hedrich, R and Hasezawa, S}, biburl = {https://www.bibsonomy.org/bibtex/2c95285b37ab488d19ea9ea8ff0039081/dirkbecker}, description = {Outward-rectifying K+ channel activities regulate cell elongation and cell division of tobacco BY-2 cells. - PubMed - NCBI}, doi = {10.1111/j.1365-313X.2008.03672.x}, interhash = {58e9e1aadfd70ccd4f3aecd8d70ff319}, intrahash = {c95285b37ab488d19ea9ea8ff0039081}, journal = {Plant J}, keywords = {myown}, month = jan, number = 1, pages = {55-64}, pmid = {18778403}, timestamp = {2017-03-21T20:39:31.000+0100}, title = {Outward-rectifying K+ channel activities regulate cell elongation and cell division of tobacco BY-2 cells}, url = {https://www.ncbi.nlm.nih.gov/pubmed/18778403}, volume = 57, year = 2009 }