%0 Journal Article %1 Deeken:2003:Plant-J:12795698 %A Deeken, R %A Ivashikina, N %A Czirjak, T %A Philippar, K %A Becker, D %A Ache, P %A Hedrich, R %D 2003 %J Plant J %K myown %N 6 %P 778-787 %T Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3 %U https://www.ncbi.nlm.nih.gov/pubmed/12795698 %V 34 %X After completion of the Arabidopsis genome-sequencing programme, crown galls induced by Agrobacterium tumefaciens may become a model system to study plant tumour development. The molecular mechanisms of nutrient supply to support tumour growth and development are still unknown. In this study, we have identified a unique profile of Shaker-like potassium channels in agrobacteria-induced Arabidopsis tumours. Comparing the gene expression pattern of rapidly growing tumours with that of non-infected tissues, we found the suppression of shoot in favour of root-specific K+ channels. Among these, the upregulation of AKT1 and AtKC1 and the suppression of AKT2/3 and GORK were most pronounced. As a consequence, K+ uptake and accumulation were elevated in the tumour (163 mm) compared to control tissues (92 mm). Patch clamp studies on tumour protoplasts identified a population expressing the electrical properties of the AKT1 K+ channel. Furthermore, plants lacking a functional AKT1 or the AKT2/3 phloem K+ channel gene did not support tumour growth. This indicates that the delivery of potassium by AKT1 and the direction of assimilates, triggered by AKT2/3, are essential for tumour growth.

@article{Deeken:2003:Plant-J:12795698, abstract = {After completion of the Arabidopsis genome-sequencing programme, crown galls induced by Agrobacterium tumefaciens may become a model system to study plant tumour development. The molecular mechanisms of nutrient supply to support tumour growth and development are still unknown. In this study, we have identified a unique profile of Shaker-like potassium channels in agrobacteria-induced Arabidopsis tumours. Comparing the gene expression pattern of rapidly growing tumours with that of non-infected tissues, we found the suppression of shoot in favour of root-specific K+ channels. Among these, the upregulation of AKT1 and AtKC1 and the suppression of AKT2/3 and GORK were most pronounced. As a consequence, K+ uptake and accumulation were elevated in the tumour (163 mm) compared to control tissues (92 mm). Patch clamp studies on tumour protoplasts identified a population expressing the electrical properties of the AKT1 K+ channel. Furthermore, plants lacking a functional AKT1 or the AKT2/3 phloem K+ channel gene did not support tumour growth. This indicates that the delivery of potassium by AKT1 and the direction of assimilates, triggered by AKT2/3, are essential for tumour growth.}, added-at = {2017-03-21T20:28:00.000+0100}, author = {Deeken, R and Ivashikina, N and Czirjak, T and Philippar, K and Becker, D and Ache, P and Hedrich, R}, biburl = {https://www.bibsonomy.org/bibtex/24c38db9a9c01022b34be2d0cfa6b87bc/dirkbecker}, description = {Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3. - PubMed - NCBI}, interhash = {b3c2d193afd2afb193e7a692bbfd0d23}, intrahash = {4c38db9a9c01022b34be2d0cfa6b87bc}, journal = {Plant J}, keywords = {myown}, month = jun, number = 6, pages = {778-787}, pmid = {12795698}, timestamp = {2017-03-21T20:28:00.000+0100}, title = {Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3}, url = {https://www.ncbi.nlm.nih.gov/pubmed/12795698}, volume = 34, year = 2003 }