Adenosine regulates the Ca2+ sensitivity of mast cell mediator release
M. Lohse, K. Klotz, M. Salzer, and U. Schwabe. Proc Natl Acad Sci U S A, 85 (23):
8875-9(December 1988)Lohse, M J Klotz, K N Salzer, M J Schwabe, U In Vitro United states
Proceedings of the National Academy of Sciences of the United States
of America Proc Natl Acad Sci U S A. 1988 Dec;85(23):8875-9..
Abstract
Mast cells release histamine and other mediators of allergy in response
to stimulation of their IgE receptors. This release is generally
thought to be mediated by an elevation of cytosolic Ca2+. Recent
evidence suggests that there might be factors that modulate the coupling
between Ca2+ levels and mediator release. The present report identifies
adenosine as one such modulator. Adenosine and several of its metabolically
stable analogues were shown to enhance histamine release from rat
peritoneal mast cells in response to stimuli such as concanavalin
A. Metabolizing endogenous adenosine with adenosine deaminase dampened
the response to stimuli, whereas trapping endogenous adenosine inside
mast cells with nucleoside-transport inhibitors markedly enhanced
stimulated histamine release. The metabolically stable adenosine
analogue 5'-(N-ethylcarboxamido)adenosine (NECA) did not affect the
initial steps in the sequence from IgE-receptor activation to mediator
release, which are generation of inositol trisphosphate and increase
of cytosolic Ca2+. However, NECA did enhance the release induced
in ATP-permeabilized cells by exogenous Ca2+, but it had no effect
on the release induced by phorbol esters. These data suggest that
adenosine sensitizes mediator release by a mechanism regulating stimulus-secretion
coupling at a step distal to receptor activation and second-messenger
generation.
Lohse, M J Klotz, K N Salzer, M J Schwabe, U In Vitro United states
Proceedings of the National Academy of Sciences of the United States
of America Proc Natl Acad Sci U S A. 1988 Dec;85(23):8875-9.
%0 Journal Article
%1 Lohse1988b
%A Lohse, M. J.
%A Klotz, K. N.
%A Salzer, M. J.
%A Schwabe, U.
%D 1988
%J Proc Natl Acad Sci U S A
%K & A/pharmacology Adenosine-5'-(N-ethylcarboxamide) Adenosine/*analogs Animals Benzofurans Calcimycin/pharmacology Calcium/*physiology Cells/drug Concanavalin Fura-2 Histamine Inositol Kinetics Male Mast Phosphates/metabolism Rats Reference Release/*drug Strains Values derivatives/pharmacology/*physiology effects effects/*physiology Mice
%N 23
%P 8875-9
%T Adenosine regulates the Ca2+ sensitivity of mast cell mediator release
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2461558
%V 85
%X Mast cells release histamine and other mediators of allergy in response
to stimulation of their IgE receptors. This release is generally
thought to be mediated by an elevation of cytosolic Ca2+. Recent
evidence suggests that there might be factors that modulate the coupling
between Ca2+ levels and mediator release. The present report identifies
adenosine as one such modulator. Adenosine and several of its metabolically
stable analogues were shown to enhance histamine release from rat
peritoneal mast cells in response to stimuli such as concanavalin
A. Metabolizing endogenous adenosine with adenosine deaminase dampened
the response to stimuli, whereas trapping endogenous adenosine inside
mast cells with nucleoside-transport inhibitors markedly enhanced
stimulated histamine release. The metabolically stable adenosine
analogue 5'-(N-ethylcarboxamido)adenosine (NECA) did not affect the
initial steps in the sequence from IgE-receptor activation to mediator
release, which are generation of inositol trisphosphate and increase
of cytosolic Ca2+. However, NECA did enhance the release induced
in ATP-permeabilized cells by exogenous Ca2+, but it had no effect
on the release induced by phorbol esters. These data suggest that
adenosine sensitizes mediator release by a mechanism regulating stimulus-secretion
coupling at a step distal to receptor activation and second-messenger
generation.
@article{Lohse1988b,
abstract = {Mast cells release histamine and other mediators of allergy in response
to stimulation of their IgE receptors. This release is generally
thought to be mediated by an elevation of cytosolic Ca2+. Recent
evidence suggests that there might be factors that modulate the coupling
between Ca2+ levels and mediator release. The present report identifies
adenosine as one such modulator. Adenosine and several of its metabolically
stable analogues were shown to enhance histamine release from rat
peritoneal mast cells in response to stimuli such as concanavalin
A. Metabolizing endogenous adenosine with adenosine deaminase dampened
the response to stimuli, whereas trapping endogenous adenosine inside
mast cells with nucleoside-transport inhibitors markedly enhanced
stimulated histamine release. The metabolically stable adenosine
analogue 5'-(N-ethylcarboxamido)adenosine (NECA) did not affect the
initial steps in the sequence from IgE-receptor activation to mediator
release, which are generation of inositol trisphosphate and increase
of cytosolic Ca2+. However, NECA did enhance the release induced
in ATP-permeabilized cells by exogenous Ca2+, but it had no effect
on the release induced by phorbol esters. These data suggest that
adenosine sensitizes mediator release by a mechanism regulating stimulus-secretion
coupling at a step distal to receptor activation and second-messenger
generation.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Lohse, M. J. and Klotz, K. N. and Salzer, M. J. and Schwabe, U.},
biburl = {https://www.bibsonomy.org/bibtex/233191e84b1ef1426178d1b530619e2b0/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {b86e3fe1402bcc5a998306876095abd2},
intrahash = {33191e84b1ef1426178d1b530619e2b0},
issn = {0027-8424 (Print) 0027-8424 (Linking)},
journal = {Proc Natl Acad Sci U S A},
keywords = {& A/pharmacology Adenosine-5'-(N-ethylcarboxamide) Adenosine/*analogs Animals Benzofurans Calcimycin/pharmacology Calcium/*physiology Cells/drug Concanavalin Fura-2 Histamine Inositol Kinetics Male Mast Phosphates/metabolism Rats Reference Release/*drug Strains Values derivatives/pharmacology/*physiology effects effects/*physiology Mice},
month = Dec,
note = {Lohse, M J Klotz, K N Salzer, M J Schwabe, U In Vitro United states
Proceedings of the National Academy of Sciences of the United States
of America Proc Natl Acad Sci U S A. 1988 Dec;85(23):8875-9.},
number = 23,
pages = {8875-9},
shorttitle = {Adenosine regulates the Ca2+ sensitivity of mast cell mediator release},
timestamp = {2010-12-14T18:22:56.000+0100},
title = {Adenosine regulates the Ca2+ sensitivity of mast cell mediator release},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2461558},
volume = 85,
year = 1988
}