%0 Journal Article %1 WOS:000329368200024 %A Melo, Mary Anne S %A Rolim, Juliana P M L %A Zanin, Iriana C J %A Silva, Jose J A %A Paschoal, Alexandre R %A Ayala, Alejandro P %A Rodrigues, Lidiany K A %C 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %D 2014 %I WILEY %J PHOTOCHEMISTRY AND PHOTOBIOLOGY %K imported %N 1 %P 183-188 %R 10.1111/php.12186 %T A Comparative Study of the Photosensitizer Penetration into Artificial Caries Lesions in Dentin Measured by the Confocal Raman Microscopy %V 90 %X This study utilized the confocal Raman microspectroscopy (CRM) technique for the first time to investigate the degree of the penetration of toluidine blue-orto (TBO) in artificial caries lesions produced by two distinct caries-inducing models. The dentin specimens (n=10) were divided into three groups: control, in vitro and in situ. Thereafter, the lesion depth and the demineralization level were evaluated by cross-sectional microhardness (CSMH). CRM mapping across the dentin surface was assessed after the dye application. The CSMH and CRM data were analyzed by t-test and ANOVA, respectively (P<0.05). The values of the lesion depth and the demineralization areas were higher for in situ samples (P<0.05). The TBO penetration values (m) for the control, in vitro and in situ groups were 44.8 +/- 5.6, 46.1 +/- 4.5 and 51.2 +/- 8.5, respectively. There were no statistically significant differences among the groups (P>0.05). The rate of TBO penetration was detected up to about <50m and the demineralization level did not influence the results. These results have showed promising parameters to develop new protocols for deep caries lesions management using photodynamic antimicrobial chemotherapy.

@article{WOS:000329368200024, abstract = {This study utilized the confocal Raman microspectroscopy (CRM) technique for the first time to investigate the degree of the penetration of toluidine blue-orto (TBO) in artificial caries lesions produced by two distinct caries-inducing models. The dentin specimens (n=10) were divided into three groups: control, in vitro and in situ. Thereafter, the lesion depth and the demineralization level were evaluated by cross-sectional microhardness (CSMH). CRM mapping across the dentin surface was assessed after the dye application. The CSMH and CRM data were analyzed by t-test and ANOVA, respectively (P<0.05). The values of the lesion depth and the demineralization areas were higher for in situ samples (P<0.05). The TBO penetration values (m) for the control, in vitro and in situ groups were 44.8 +/- 5.6, 46.1 +/- 4.5 and 51.2 +/- 8.5, respectively. There were no statistically significant differences among the groups (P>0.05). The rate of TBO penetration was detected up to about <50m and the demineralization level did not influence the results. These results have showed promising parameters to develop new protocols for deep caries lesions management using photodynamic antimicrobial chemotherapy.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA}, author = {Melo, Mary Anne S and Rolim, Juliana P M L and Zanin, Iriana C J and Silva, Jose J A and Paschoal, Alexandre R and Ayala, Alejandro P and Rodrigues, Lidiany K A}, biburl = {https://www.bibsonomy.org/bibtex/26adbbd8970db6c08b27284857fbcf7d6/ppgfis_ufc_br}, doi = {10.1111/php.12186}, interhash = {cc0d331464c08621b39b20ba500c006b}, intrahash = {6adbbd8970db6c08b27284857fbcf7d6}, issn = {0031-8655}, journal = {PHOTOCHEMISTRY AND PHOTOBIOLOGY}, keywords = {imported}, number = 1, pages = {183-188}, publisher = {WILEY}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {A Comparative Study of the Photosensitizer Penetration into Artificial Caries Lesions in Dentin Measured by the Confocal Raman Microscopy}, tppubtype = {article}, volume = 90, year = 2014 }