This study utilized the confocal Raman microspectroscopy (CRM) technique
for the first time to investigate the degree of the penetration of
toluidine blue-orto (TBO) in artificial caries lesions produced by two distinct caries-inducing models. The dentin specimens (n=10) were
divided into three groups: control, in vitro and in situ. Thereafter,
the lesion depth and the demineralization level were evaluated by
cross-sectional microhardness (CSMH). CRM mapping across the dentin
surface was assessed after the dye application. The CSMH and CRM data
were analyzed by t-test and ANOVA, respectively (P<0.05). The values of
the lesion depth and the demineralization areas were higher for in situ
samples (P<0.05). The TBO penetration values (m) for the control, in
vitro and in situ groups were 44.8 +/- 5.6, 46.1 +/- 4.5 and 51.2 +/-
8.5, respectively. There were no statistically significant differences
among the groups (P>0.05). The rate of TBO penetration was detected up
to about <50m and the demineralization level did not influence the
results. These results have showed promising parameters to develop new
protocols for deep caries lesions management using photodynamic
antimicrobial chemotherapy.
%0 Journal Article
%1 WOS:000329368200024
%A Melo, Mary Anne S
%A Rolim, Juliana P M L
%A Zanin, Iriana C J
%A Silva, Jose J A
%A Paschoal, Alexandre R
%A Ayala, Alejandro P
%A Rodrigues, Lidiany K A
%C 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
%D 2014
%I WILEY
%J PHOTOCHEMISTRY AND PHOTOBIOLOGY
%K imported
%N 1
%P 183-188
%R 10.1111/php.12186
%T A Comparative Study of the Photosensitizer Penetration into Artificial
Caries Lesions in Dentin Measured by the Confocal Raman Microscopy
%V 90
%X This study utilized the confocal Raman microspectroscopy (CRM) technique
for the first time to investigate the degree of the penetration of
toluidine blue-orto (TBO) in artificial caries lesions produced by two distinct caries-inducing models. The dentin specimens (n=10) were
divided into three groups: control, in vitro and in situ. Thereafter,
the lesion depth and the demineralization level were evaluated by
cross-sectional microhardness (CSMH). CRM mapping across the dentin
surface was assessed after the dye application. The CSMH and CRM data
were analyzed by t-test and ANOVA, respectively (P<0.05). The values of
the lesion depth and the demineralization areas were higher for in situ
samples (P<0.05). The TBO penetration values (m) for the control, in
vitro and in situ groups were 44.8 +/- 5.6, 46.1 +/- 4.5 and 51.2 +/-
8.5, respectively. There were no statistically significant differences
among the groups (P>0.05). The rate of TBO penetration was detected up
to about <50m and the demineralization level did not influence the
results. These results have showed promising parameters to develop new
protocols for deep caries lesions management using photodynamic
antimicrobial chemotherapy.
@article{WOS:000329368200024,
abstract = {This study utilized the confocal Raman microspectroscopy (CRM) technique
for the first time to investigate the degree of the penetration of
toluidine blue-orto (TBO) in artificial caries lesions produced by two distinct caries-inducing models. The dentin specimens (n=10) were
divided into three groups: control, in vitro and in situ. Thereafter,
the lesion depth and the demineralization level were evaluated by
cross-sectional microhardness (CSMH). CRM mapping across the dentin
surface was assessed after the dye application. The CSMH and CRM data
were analyzed by t-test and ANOVA, respectively (P<0.05). The values of
the lesion depth and the demineralization areas were higher for in situ
samples (P<0.05). The TBO penetration values (m) for the control, in
vitro and in situ groups were 44.8 +/- 5.6, 46.1 +/- 4.5 and 51.2 +/-
8.5, respectively. There were no statistically significant differences
among the groups (P>0.05). The rate of TBO penetration was detected up
to about <50m and the demineralization level did not influence the
results. These results have showed promising parameters to develop new
protocols for deep caries lesions management using photodynamic
antimicrobial chemotherapy.},
added-at = {2022-05-23T20:00:14.000+0200},
address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA},
author = {Melo, Mary Anne S and Rolim, Juliana P M L and Zanin, Iriana C J and Silva, Jose J A and Paschoal, Alexandre R and Ayala, Alejandro P and Rodrigues, Lidiany K A},
biburl = {https://www.bibsonomy.org/bibtex/26adbbd8970db6c08b27284857fbcf7d6/ppgfis_ufc_br},
doi = {10.1111/php.12186},
interhash = {cc0d331464c08621b39b20ba500c006b},
intrahash = {6adbbd8970db6c08b27284857fbcf7d6},
issn = {0031-8655},
journal = {PHOTOCHEMISTRY AND PHOTOBIOLOGY},
keywords = {imported},
number = 1,
pages = {183-188},
publisher = {WILEY},
pubstate = {published},
timestamp = {2022-05-23T20:00:14.000+0200},
title = {A Comparative Study of the Photosensitizer Penetration into Artificial
Caries Lesions in Dentin Measured by the Confocal Raman Microscopy},
tppubtype = {article},
volume = 90,
year = 2014
}