A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.
%0 Journal Article
%1 wadowsky_inhibition_1994-1
%A Wadowsky, R M
%A Laus, S
%A Libert, T
%A States, S J
%A Ehrlich, G D
%D 1994
%J Journal of Clinical Microbiology
%K Acid, Acids, Alginates, Aluminum, Bacterial, Bacteriological Base Bordetella Chain Cough Data, Glucuronic Hexuronic Humans, Molecular Nasopharynx, Polyethylene Polymerase Probes, Reaction, Sequence Sequence, Techniques, Terephthalates, Whooping pertussis, {DNA,} {DNA}
%N 4
%P 1054--1057
%T Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab
%U http://www.ncbi.nlm.nih.gov/pubmed/8027309
%V 32
%X A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.
@article{wadowsky_inhibition_1994-1,
abstract = {A {PCR-based} assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) {CFU} of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in {PCR-based} assays.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Wadowsky, R M and Laus, S and Libert, T and States, S J and Ehrlich, G D},
biburl = {https://www.bibsonomy.org/bibtex/28d24f52b761804c5a5484d908b6c4cda/jelias},
interhash = {d083c10e81c59a79b0c9d4cdc9a5fec0},
intrahash = {8d24f52b761804c5a5484d908b6c4cda},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
keywords = {Acid, Acids, Alginates, Aluminum, Bacterial, Bacteriological Base Bordetella Chain Cough Data, Glucuronic Hexuronic Humans, Molecular Nasopharynx, Polyethylene Polymerase Probes, Reaction, Sequence Sequence, Techniques, Terephthalates, Whooping pertussis, {DNA,} {DNA}},
month = apr,
note = {{PMID:} 8027309},
number = 4,
pages = {1054--1057},
timestamp = {2011-03-11T10:06:45.000+0100},
title = {Inhibition of {PCR-based} assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab},
url = {http://www.ncbi.nlm.nih.gov/pubmed/8027309},
volume = 32,
year = 1994
}