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Dissecting self-renewal in stem cells with RNA interference

by: Ivanova, R Dobrin, R Lu, I Kotenko, J Levorse, C DeCoste, X Schafer, Y Lun, and I R Lemischka

In: Nature, Vol. 442, Nr. 7102 (August 2006) , p. 533-538.

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URL:	http://www.ncbi.nlm.nih.gov/pubmed/16767105
DOI:	10.1038/nature04915

We present an integrated approach to identify genetic mechanisms that control self-renewal in mouse embryonic stem cells. We use short hairpin RNA shRNA loss-of-function techniques to downregulate a set of gene products whose expression patterns suggest self-renewal regulatory functions. We focus on transcriptional regulators and identify seven genes for which shRNA-mediated depletion negatively affects self-renewal, including four genes with previously unrecognized roles in self-renewal. Perturbations of these gene products are combined with dynamic, global analyses of gene expression. Our studies suggest specific biological roles for these molecules and reveal the complexity of cell fate regulation in embryonic stem cells.

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Dissecting self-renewal in stem cells with RNA int... [Nature. 2006] - PubMed result

BibTeX record

@article{Ivanova:2006:Nature:16767105,
  abstract = {We present an integrated approach to identify genetic mechanisms that control self-renewal in mouse embryonic stem cells. We use short hairpin RNA (shRNA) loss-of-function techniques to downregulate a set of gene products whose expression patterns suggest self-renewal regulatory functions. We focus on transcriptional regulators and identify seven genes for which shRNA-mediated depletion negatively affects self-renewal, including four genes with previously unrecognized roles in self-renewal. Perturbations of these gene products are combined with dynamic, global analyses of gene expression. Our studies suggest specific biological roles for these molecules and reveal the complexity of cell fate regulation in embryonic stem cells.},
  added-at = {2009-11-19T21:48:01.000+0100},
  author = {Ivanova and Dobrin, R and Lu, R and Kotenko, I and Levorse, J and DeCoste, C and Schafer, X and Lun, Y and Lemischka, I R},
  biburl = {http://www.bibsonomy.org/bibtex/20f03906b03282aa2ebf1d60b0ae44574/cchen63},
  description = {Dissecting self-renewal in stem cells with RNA int... [Nature. 2006] - PubMed result},
  doi = {10.1038/nature04915},
  interhash = {d47179d6f9eeef372c9393539ab864a7},
  intrahash = {0f03906b03282aa2ebf1d60b0ae44574},
  journal = {Nature},
  keywords = {imported},
  month = Aug,
  number = 7102,
  pages = {533-538},
  pmid = {16767105},
  timestamp = {2009-11-19T21:48:01.000+0100},
  title = {Dissecting self-renewal in stem cells with RNA interference},
  url = {http://www.ncbi.nlm.nih.gov/pubmed/16767105},
  volume = 442,
  year = 2006
}

Endnote record

%0 Journal Article
%1 Ivanova:2006:Nature:16767105
%A Ivanova, 
%A Dobrin, R
%A Lu, R
%A Kotenko, I
%A Levorse, J
%A DeCoste, C
%A Schafer, X
%A Lun, Y
%A Lemischka, I R
%D 2006
%J Nature
%K 
%N 7102
%P 533-538
%T Dissecting self-renewal in stem cells with RNA interference
%R 10.1038/nature04915
%U http://www.ncbi.nlm.nih.gov/pubmed/16767105
%V 442
%X We present an integrated approach to identify genetic mechanisms that control self-renewal in mouse embryonic stem cells. We use short hairpin RNA (shRNA) loss-of-function techniques to downregulate a set of gene products whose expression patterns suggest self-renewal regulatory functions. We focus on transcriptional regulators and identify seven genes for which shRNA-mediated depletion negatively affects self-renewal, including four genes with previously unrecognized roles in self-renewal. Perturbations of these gene products are combined with dynamic, global analyses of gene expression. Our studies suggest specific biological roles for these molecules and reveal the complexity of cell fate regulation in embryonic stem cells.