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Abstract

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

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A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. - PubMed - NCBI

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Fisher:2011:Genome-Biol:21205303
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