Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
Описание
A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. - PubMed - NCBI
%0 Journal Article
%1 Fisher:2011:Genome-Biol:21205303
%A Fisher, S
%A Barry, A
%A Abreu, J
%A Minie, B
%A Nolan, J
%A Delorey, T M
%A Young, G
%A Fennell, T J
%A Allen, A
%A Ambrogio, L
%A Berlin, A M
%A Blumenstiel, B
%A Cibulskis, K
%A Friedrich, D
%A Johnson, R
%A Juhn, F
%A Reilly, B
%A Shammas, R
%A Stalker, J
%A Sykes, S M
%A Thompson, J
%A Walsh, J
%A Zimmer, A
%A Zwirko, Z
%A Gabriel, S
%A Nicol, R
%A Nusbaum, C
%D 2011
%J Genome Biol
%K MUSTREAD broad exome fulltext methods next-generation-sequencing sureselect wes
%N 1
%R 10.1186/gb-2011-12-1-r1
%T A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
%U https://www.ncbi.nlm.nih.gov/pubmed/21205303/
%V 12
%X Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
@article{Fisher:2011:Genome-Biol:21205303,
abstract = {Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.},
added-at = {2017-10-03T14:07:45.000+0200},
author = {Fisher, S and Barry, A and Abreu, J and Minie, B and Nolan, J and Delorey, T M and Young, G and Fennell, T J and Allen, A and Ambrogio, L and Berlin, A M and Blumenstiel, B and Cibulskis, K and Friedrich, D and Johnson, R and Juhn, F and Reilly, B and Shammas, R and Stalker, J and Sykes, S M and Thompson, J and Walsh, J and Zimmer, A and Zwirko, Z and Gabriel, S and Nicol, R and Nusbaum, C},
biburl = {https://www.bibsonomy.org/bibtex/2101e25516c70e4b7ceedca12c54a0d2f/marcsaric},
description = {A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. - PubMed - NCBI},
doi = {10.1186/gb-2011-12-1-r1},
interhash = {1e7087079eb391f3dcd41e954d4aa443},
intrahash = {101e25516c70e4b7ceedca12c54a0d2f},
journal = {Genome Biol},
keywords = {MUSTREAD broad exome fulltext methods next-generation-sequencing sureselect wes},
number = 1,
pmid = {21205303},
timestamp = {2017-10-03T14:07:45.000+0200},
title = {A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries},
url = {https://www.ncbi.nlm.nih.gov/pubmed/21205303/},
volume = 12,
year = 2011
}