The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.
%0 Journal Article
%1 RN62
%A Ravanal, M.C.
%A Espinosa, Y.
%A Rosa, L.
%A Vaca, I.
%A Polanco, R.
%A Eyzaguirre, J.
%A Levican, G.
%A Chavez, R.
%D 2012
%J Mycoscience
%K cloning dqcauchile expression, genes, glucose heterologous induction, penicillium purpurogenum, secretion, transformation, trichoderma-reesei, xylanase,
%N 2
%P 152-155
%R 10.1007/s10267-011-0144-1
%T Glucose-Induced Production of a Penicillium Purpurogenum Xylanase by Aspergillus Nidulans
%U /brokenurl#<Go to ISI>://WOS:000301638800010
%V 53
%X The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.
@article{RN62,
abstract = {The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0.72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.},
added-at = {2019-12-04T03:57:35.000+0100},
author = {Ravanal, M.C. and Espinosa, Y. and Rosa, L. and Vaca, I. and Polanco, R. and Eyzaguirre, J. and Levican, G. and Chavez, R.},
biburl = {https://www.bibsonomy.org/bibtex/21c9e182bbb8104a15e14baf3637f5a1a/dqcauchile},
doi = {10.1007/s10267-011-0144-1},
interhash = {073e172b9af0a5ece4299384ffcc462d},
intrahash = {1c9e182bbb8104a15e14baf3637f5a1a},
issn = {1340-3540},
journal = {Mycoscience},
keywords = {cloning dqcauchile expression, genes, glucose heterologous induction, penicillium purpurogenum, secretion, transformation, trichoderma-reesei, xylanase,},
number = 2,
pages = {152-155},
timestamp = {2019-12-04T03:58:17.000+0100},
title = {Glucose-Induced Production of a Penicillium Purpurogenum Xylanase by Aspergillus Nidulans},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000301638800010},
volume = 53,
year = 2012
}