Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods
The VITEK 2 AST-N111 card was evaluated for detection of extended-spectrum beta-lactamases (ESBLs) by testing 51 ESBL positive and 50 ESBL negative isolates of E. coli, K. pneumoniae, and K. oxytoca. The occurrence of beta-lactamase genes was confirmed by PCR and sequencing. The advanced expert system (AES) of the VITEK 2 system achieved sensitivity and specificity values of 100\% and 96.0\%, respectively. The ESBL test of the VITEK 2 AST-N111 card showed a sensitivity of 92.1\% and a specificity of 90.0\%. Contradictory results obtained with the two VITEK 2 tools could be clarified by combination disk tests in nine of 11 isolates. The combined use of AES and ESBL tests of the AST-N111 card in association with combination disk tests in case of contradictory results seems to be a reliable method for ESBL detection.
%0 Journal Article
%1 valenza_evaluation_2011
%A Valenza, G
%A Müller, S
%A Schmitt, C
%A Turnwald, D
%A Lam, T-T
%A Frosch, M
%A Abele-Horn, M
%A Pfeifer, Y
%D 2011
%J European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology
%K ag_horn imported
%R 10.1007/s10096-011-1169-2
%T Evaluation of the VITEK 2 AST-N111 card for detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to ESBL Etests and combination disk methods
%U http://www.ncbi.nlm.nih.gov/pubmed/21271268
%X The VITEK 2 AST-N111 card was evaluated for detection of extended-spectrum beta-lactamases (ESBLs) by testing 51 ESBL positive and 50 ESBL negative isolates of E. coli, K. pneumoniae, and K. oxytoca. The occurrence of beta-lactamase genes was confirmed by PCR and sequencing. The advanced expert system (AES) of the VITEK 2 system achieved sensitivity and specificity values of 100\% and 96.0\%, respectively. The ESBL test of the VITEK 2 AST-N111 card showed a sensitivity of 92.1\% and a specificity of 90.0\%. Contradictory results obtained with the two VITEK 2 tools could be clarified by combination disk tests in nine of 11 isolates. The combined use of AES and ESBL tests of the AST-N111 card in association with combination disk tests in case of contradictory results seems to be a reliable method for ESBL detection.
@article{valenza_evaluation_2011,
abstract = {The {VITEK} 2 {AST-N111} card was evaluated for detection of extended-spectrum beta-lactamases {(ESBLs)} by testing 51 {ESBL} positive and 50 {ESBL} negative isolates of E. coli, K. pneumoniae, and K. oxytoca. The occurrence of beta-lactamase genes was confirmed by {PCR} and sequencing. The advanced expert system {(AES)} of the {VITEK} 2 system achieved sensitivity and specificity values of 100\% and 96.0\%, respectively. The {ESBL} test of the {VITEK} 2 {AST-N111} card showed a sensitivity of 92.1\% and a specificity of 90.0\%. Contradictory results obtained with the two {VITEK} 2 tools could be clarified by combination disk tests in nine of 11 isolates. The combined use of {AES} and {ESBL} tests of the {AST-N111} card in association with combination disk tests in case of contradictory results seems to be a reliable method for {ESBL} detection.},
added-at = {2011-04-07T15:44:20.000+0200},
author = {Valenza, G and Müller, S and Schmitt, C and Turnwald, D and Lam, {T-T} and Frosch, M and {Abele-Horn}, M and Pfeifer, Y},
biburl = {https://www.bibsonomy.org/bibtex/21db7b1f5f3d1f9a323d162deb42ab86a/hymi},
doi = {10.1007/s10096-011-1169-2},
interhash = {3132789c7918f32864b1f2cc4640ba3c},
intrahash = {1db7b1f5f3d1f9a323d162deb42ab86a},
issn = {1435-4373},
journal = {European Journal of Clinical Microbiology \& Infectious Diseases: Official Publication of the European Society of Clinical Microbiology},
keywords = {ag_horn imported},
month = jan,
note = {{PMID:} 21271268},
timestamp = {2011-04-07T15:47:41.000+0200},
title = {Evaluation of the {VITEK} 2 {AST-N111} card for detection of extended-spectrum beta-lactamases {(ESBLs)} in Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca compared to {ESBL} Etests and combination disk methods},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21271268},
year = 2011
}