Alginate is a family of polysaccharides isolated from seaweeds or
produced by certain bacteria composed of (1 --> 4)-linked residues
of alpha-L-guluronic acid (G) and beta-D-mannuronic acid (M) varying
both in abundance and sequence along the chain. Chain sequences from
five different sources were reconstructed by using experimentally
determined fractions of the eight possible triad arrangements as
parameters in second-order Markov chains. The Monte Carlo generated
chain sequences were analyzed with respect to gelling ability, assuming
a cooperative model for junction formation. The cooperative model
was approximated by a step function at LG(min) representing the minimum
level of G blocks being able to form a junction. By varying LG(min)
from 4 to 12, it was found that there was a fraction, the loose-end
fraction, of chains within each sample that had less than two possible
junction zones. The loose-end fraction was calculated to decrease
with increasing chain length and with decreasing LG(min). The gel
strength was found to correlate to the calculated number of elastically
active chains. The sol fraction constitutes chains with no G blocks
of length exceeding LG(min). The sol fraction was found to depend
on the source of the alginate, to decrease with increasing chain
length and decreasing LG(min), and to constitute approximately 35-50%
of the loose-end fraction. The sol fraction was further predicted
to be enriched in beta-D-mannuronic acid residues, the enrichment
being larger for smaller LG(min). The model unites properties at
the polymer level to those of practical interest, such as gel strength
and porosity and enrichment of beta-D-mannuronic acid in leaking
material, properties that are of prime importance when applying alginates
as immobilization material for implantation purposes.
%0 Journal Article
%1 STOKKE1991
%A STOKKE, B. T.
%A SMIDSROD, O.
%A BRUHEIM, P.
%A SKJAKBRAEK, G.
%C 1155 16TH ST, NW, WASHINGTON, DC 20036
%D 1991
%I AMER CHEMICAL SOC
%J Macromolecules
%K ;; [ISI:] dependence; gels; state
%N 16
%P 4637 -- 4645
%T DISTRIBUTION OF URONATE RESIDUES IN ALGINATE CHAINS IN RELATION TO
ALGINATE GELLING PROPERTIES
%V 24
%X Alginate is a family of polysaccharides isolated from seaweeds or
produced by certain bacteria composed of (1 --> 4)-linked residues
of alpha-L-guluronic acid (G) and beta-D-mannuronic acid (M) varying
both in abundance and sequence along the chain. Chain sequences from
five different sources were reconstructed by using experimentally
determined fractions of the eight possible triad arrangements as
parameters in second-order Markov chains. The Monte Carlo generated
chain sequences were analyzed with respect to gelling ability, assuming
a cooperative model for junction formation. The cooperative model
was approximated by a step function at LG(min) representing the minimum
level of G blocks being able to form a junction. By varying LG(min)
from 4 to 12, it was found that there was a fraction, the loose-end
fraction, of chains within each sample that had less than two possible
junction zones. The loose-end fraction was calculated to decrease
with increasing chain length and with decreasing LG(min). The gel
strength was found to correlate to the calculated number of elastically
active chains. The sol fraction constitutes chains with no G blocks
of length exceeding LG(min). The sol fraction was found to depend
on the source of the alginate, to decrease with increasing chain
length and decreasing LG(min), and to constitute approximately 35-50%
of the loose-end fraction. The sol fraction was further predicted
to be enriched in beta-D-mannuronic acid residues, the enrichment
being larger for smaller LG(min). The model unites properties at
the polymer level to those of practical interest, such as gel strength
and porosity and enrichment of beta-D-mannuronic acid in leaking
material, properties that are of prime importance when applying alginates
as immobilization material for implantation purposes.
@article{STOKKE1991,
__markedentry = {[phpts:6]},
abstract = {Alginate is a family of polysaccharides isolated from seaweeds or
produced by certain bacteria composed of (1 --> 4)-linked residues
of alpha-L-guluronic acid (G) and beta-D-mannuronic acid (M) varying
both in abundance and sequence along the chain. Chain sequences from
five different sources were reconstructed by using experimentally
determined fractions of the eight possible triad arrangements as
parameters in second-order Markov chains. The Monte Carlo generated
chain sequences were analyzed with respect to gelling ability, assuming
a cooperative model for junction formation. The cooperative model
was approximated by a step function at LG(min) representing the minimum
level of G blocks being able to form a junction. By varying LG(min)
from 4 to 12, it was found that there was a fraction, the loose-end
fraction, of chains within each sample that had less than two possible
junction zones. The loose-end fraction was calculated to decrease
with increasing chain length and with decreasing LG(min). The gel
strength was found to correlate to the calculated number of elastically
active chains. The sol fraction constitutes chains with no G blocks
of length exceeding LG(min). The sol fraction was found to depend
on the source of the alginate, to decrease with increasing chain
length and decreasing LG(min), and to constitute approximately 35-50%
of the loose-end fraction. The sol fraction was further predicted
to be enriched in beta-D-mannuronic acid residues, the enrichment
being larger for smaller LG(min). The model unites properties at
the polymer level to those of practical interest, such as gel strength
and porosity and enrichment of beta-D-mannuronic acid in leaking
material, properties that are of prime importance when applying alginates
as immobilization material for implantation purposes.},
added-at = {2011-11-04T13:47:04.000+0100},
address = {1155 16TH ST, NW, WASHINGTON, DC 20036},
author = {STOKKE, B. T. and SMIDSROD, O. and BRUHEIM, P. and SKJAKBRAEK, G.},
authoraddress = {UNIV TRONDHEIM,NTH,DIV BIOTECHNOL,N-7034 TRONDHEIM,NORWAY.},
biburl = {https://www.bibsonomy.org/bibtex/22f375f798a48b38c6aeb9b0a2e1c101d/pawelsikorski},
citedref = {ANDRESEN IL, 1977, ACS SYM SER, V48, P361 ; ANDRESEN IL, 1977, CARBOHYD
RES, V58, P271 ; BAILEY E, 1977, COLLOID POLYM SCI, V255, P856 ;
CANTOR CR, 1980, BIOPHYSICAL CHEM, V3 ; CESARO A, 1982, THERM ANAL,
V2, P815 ; DUBOIS M, 1956, ANAL CHEM, V28, P350 ; GRANT GT, 1973,
FEBS LETT, V32, P195 ; GRASDALEN H, 1979, CARBOHYD RES, V68, P21
; GRASDALEN H, 1981, CARBOHYD RES, V89, P179 ; GRASDALEN H, 1983,
CARBOHYD RES, V118, P255 ; HALLMAN GM, 1973, MACROMOLECULES, V6,
P386 ; HAUG A, 1964, 30 NORW I SEAW RES R ; HAUGEN F, 1990, CARBOHYD
RES, V198, P101 ; HEYRAUD A, 1990, FOOD HYDROCOLLOIDS, V4, P59 ;
KOHN R, 1968, ACTA CHEM SCAND, V22, P3098 ; KOHN R, 1975, PURE APPL
CHEM, V42, P371 ; LAPASIN R, 1988, PROGR TRENDS RHEOLOG, V2, P422
; LARSEN B, 1969, ACTA CHEM SCAND, V23, P355 ; LARSEN B, 1970, ACTA
CHEM SCAND, V23, P726 ; LARSEN B, 1981, 10TH P INT SEAW S, P7 ; LARSEN
B, 1986, CARBOHYD RES, V146, P342 ; MARTINSEN A, 1989, BIOTECHNOL
BIOENG, V33, P79 ; MARTINSEN A, 1990, THESIS U TRONDHEIM N ; MITCHELL
JR, 1976, J TEXTURE STUD, V7, P313 ; MYHRE JM, 1970, MARKOV CHAINS
MONTE, P13 ; OTTERLEI M, IN PRESS J IMMUNTHER ; PELLER L, 1963, J
CHEM PHYS, V36, P2976 ; PRICE FP, 1970, MARKOV CHAINS MONTE, P187
; REES DA, 1981, PURE APPL CHEM, V53, P1 ; SEGEREN AJM, 1974, DISCUSS
CHEM SOC, V57, P255 ; SKJAKBRAEK G, 1986, INT J BIOL MACROMOL, V8,
P330 ; SKJAKBRAEK G, 1988, THESIS U TRONDHEIM N ; SKJAKBRAEK G, 1989,
CARBOHYD POLYM, V10, P31 ; SMIDSROD O, 1969, MACROMOLECULES, V2,
P42 ; SMIDSROD O, 1972, ACTA CHEM SCAND, V26, P2063 ; SMIDSROD O,
1972, ACTA CHEM SCAND, V26, P79 ; SMIDSROD O, 1973, 34 NORW I SEAW
RES R ; SMIDSROD O, 1974, FARADAY DISCUSS, V57, P263 ; SMIDSROD O,
1990, TRENDS BIOTECHNOL, V8, P71},
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intrahash = {2f375f798a48b38c6aeb9b0a2e1c101d},
isifile-dt = {Article},
isifile-ga = {GA151},
isifile-j9 = {MACROMOLECULES},
isifile-nr = {39},
isifile-pi = {WASHINGTON},
isifile-rp = {STOKKE, BT, UNIV TRONDHEIM,NTH,DEPT PHYS,NORWEGIAN BIOPOLYMER ; LAB,N-7034
TRONDHEIM,NORWAY.},
isifile-sc = {Polymer Science},
isifile-tc = {48},
issn = {0024-9297},
journal = {Macromolecules},
keywords = {;; [ISI:] dependence; gels; state},
language = {English},
month = {AUG 5},
number = 16,
owner = {phpts},
pages = {4637 -- 4645},
publisher = {AMER CHEMICAL SOC},
size = {9 p.},
sourceid = {ISI:A1991GA15100026},
timestamp = {2011-11-04T13:47:24.000+0100},
title = {DISTRIBUTION OF URONATE RESIDUES IN ALGINATE CHAINS IN RELATION TO
ALGINATE GELLING PROPERTIES},
volume = 24,
year = 1991
}