Three non-ribosomal peptide synthetase genes, termed sidD, sidC and
sidE, have been identified in Aspergillus fumigatus. Gene expression
analysis by RT-PCR confirms that expression of both sidD and C was
reduced by up to 90% under iron-replete conditions indicative of
a likely role in siderophore biosynthesis. SidE expression was less
sensitive to iron levels. In addition, two proteins purified from
mycelia grown under iron-limiting conditions corresponded to SidD
( approximately 200 kDa) and SidC (496 kDa) as determined by MALDI
ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore
synthetases are unique in bacteria and fungi and represent an attractive
target for antimicrobial chemotherapy.
%0 Journal Article
%1 citeulike:498212
%A Reiber, K.
%A Reeves, E. P.
%A Neville, C. M.
%A Winkler, R.
%A Gebhardt, P.
%A Kavanagh, K.
%A Doyle, S.
%C National Institute for Cellular Biotechnology, Department of Biology,
National University of Ireland, Maynooth, Co. Kildare, Ireland.
%D 2005
%J FEMS Microbiol Lett
%K siderophore nrps maldi
%N 1
%P 83--91
%R 10.1016/j.femsle.2005.05.028
%T The expression of selected non-ribosomal peptide synthetases in Aspergillus
fumigatus is controlled by the availability of free iron.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15953695
%V 248
%X Three non-ribosomal peptide synthetase genes, termed sidD, sidC and
sidE, have been identified in Aspergillus fumigatus. Gene expression
analysis by RT-PCR confirms that expression of both sidD and C was
reduced by up to 90% under iron-replete conditions indicative of
a likely role in siderophore biosynthesis. SidE expression was less
sensitive to iron levels. In addition, two proteins purified from
mycelia grown under iron-limiting conditions corresponded to SidD
( approximately 200 kDa) and SidC (496 kDa) as determined by MALDI
ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore
synthetases are unique in bacteria and fungi and represent an attractive
target for antimicrobial chemotherapy.
@article{citeulike:498212,
abstract = {Three non-ribosomal peptide synthetase genes, termed sidD, sidC and
sidE, have been identified in Aspergillus fumigatus. Gene expression
analysis by RT-PCR confirms that expression of both sidD and C was
reduced by up to 90% under iron-replete conditions indicative of
a likely role in siderophore biosynthesis. SidE expression was less
sensitive to iron levels. In addition, two proteins purified from
mycelia grown under iron-limiting conditions corresponded to SidD
( approximately 200 kDa) and SidC (496 kDa) as determined by MALDI
ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore
synthetases are unique in bacteria and fungi and represent an attractive
target for antimicrobial chemotherapy.},
added-at = {2007-02-02T11:54:15.000+0100},
address = {National Institute for Cellular Biotechnology, Department of Biology,
National University of Ireland, Maynooth, Co. Kildare, Ireland.},
author = {Reiber, K. and Reeves, E. P. and Neville, C. M. and Winkler, R. and Gebhardt, P. and Kavanagh, K. and Doyle, S.},
biburl = {https://www.bibsonomy.org/bibtex/23474a9c07725406da0d07ebc5dbdbed7/robert},
citeulike-article-id = {498212},
doi = {10.1016/j.femsle.2005.05.028},
interhash = {ef9e70759dfaaa413d03fc3b7106e492},
intrahash = {3474a9c07725406da0d07ebc5dbdbed7},
issn = {0378-1097},
journal = {FEMS Microbiol Lett},
keywords = {siderophore nrps maldi},
month = {July},
number = 1,
pages = {83--91},
priority = {0},
timestamp = {2007-02-02T11:54:15.000+0100},
title = {The expression of selected non-ribosomal peptide synthetases in \textit{Aspergillus
fumigatus} is controlled by the availability of free iron.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=15953695},
volume = 248,
year = 2005
}