Cell type-specific regulation of beta2-adrenoceptor mRNA by agonists
S. Danner, and M. Lohse. Eur J Pharmacol, 331 (1):
73-8(July 1997)Danner, S Lohse, M J Comparative Study Research Support, Non-U.S.
Gov't Netherlands European journal of pharmacology Eur J Pharmacol.
1997 Jul 16;331(1):73-8..
Abstract
Prolonged agonist stimulation of beta2-adrenoceptors results in receptor
down-regulation which is often paralleled by a reduction of the corresponding
mRNA. In this study, we investigated the agonist-dependent regulation
of beta2-adrenoceptor mRNA in DDT1-MF2 smooth muscle cells and C6
glioma cells. In DDT1-MF2 cells the half-life of the mRNA was 12
h in monolayer compared to 2 h in suspension cultures. Under both
conditions, the agonist isoproterenol reduced this half-life by a
factor of 2. In contrast, in C6 glioma cells isoproterenol had no
effect on the mRNA stability, even though it reduced mRNA levels
by approximately 50%. Isoproterenol-induced downregulation of beta2-adrenoceptor
mRNA was completely blocked in C6 cells by the presence of a protein
synthesis inhibitor, while this was not so in DDT1-MF2-cells. These
data show that beta2-adrenoceptor downregulation occurs via cell-type
specific mechanisms.
Danner, S Lohse, M J Comparative Study Research Support, Non-U.S.
Gov't Netherlands European journal of pharmacology Eur J Pharmacol.
1997 Jul 16;331(1):73-8.
%0 Journal Article
%1 Danner1997
%A Danner, S.
%A Lohse, M. J.
%D 1997
%J Eur J Pharmacol
%K & Adrenergic Animals Assay Blotting, Cell Chain Cricetinae Cultured Cycle Down-Regulation Exotoxins/pharmacology Expression Gene Inhibitors/pharmacology Isoproterenol/antagonists Line Messenger/*analysis Northern Polymerase Protein RNA, Radioligand Rats Reaction Regulation Synthesis Tumor beta-Agonists/*pharmacology beta/*genetics inhibitors/pharmacology Receptor
%N 1
%P 73-8
%T Cell type-specific regulation of beta2-adrenoceptor mRNA by agonists
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9274932
%V 331
%X Prolonged agonist stimulation of beta2-adrenoceptors results in receptor
down-regulation which is often paralleled by a reduction of the corresponding
mRNA. In this study, we investigated the agonist-dependent regulation
of beta2-adrenoceptor mRNA in DDT1-MF2 smooth muscle cells and C6
glioma cells. In DDT1-MF2 cells the half-life of the mRNA was 12
h in monolayer compared to 2 h in suspension cultures. Under both
conditions, the agonist isoproterenol reduced this half-life by a
factor of 2. In contrast, in C6 glioma cells isoproterenol had no
effect on the mRNA stability, even though it reduced mRNA levels
by approximately 50%. Isoproterenol-induced downregulation of beta2-adrenoceptor
mRNA was completely blocked in C6 cells by the presence of a protein
synthesis inhibitor, while this was not so in DDT1-MF2-cells. These
data show that beta2-adrenoceptor downregulation occurs via cell-type
specific mechanisms.
@article{Danner1997,
abstract = {Prolonged agonist stimulation of beta2-adrenoceptors results in receptor
down-regulation which is often paralleled by a reduction of the corresponding
mRNA. In this study, we investigated the agonist-dependent regulation
of beta2-adrenoceptor mRNA in DDT1-MF2 smooth muscle cells and C6
glioma cells. In DDT1-MF2 cells the half-life of the mRNA was 12
h in monolayer compared to 2 h in suspension cultures. Under both
conditions, the agonist isoproterenol reduced this half-life by a
factor of 2. In contrast, in C6 glioma cells isoproterenol had no
effect on the mRNA stability, even though it reduced mRNA levels
by approximately 50%. Isoproterenol-induced downregulation of beta2-adrenoceptor
mRNA was completely blocked in C6 cells by the presence of a protein
synthesis inhibitor, while this was not so in DDT1-MF2-cells. These
data show that beta2-adrenoceptor downregulation occurs via cell-type
specific mechanisms.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Danner, S. and Lohse, M. J.},
biburl = {https://www.bibsonomy.org/bibtex/23cd605d75291fe2e1dd96525760e2c05/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {c8b4d1996e771d351d7691eb5300c2c6},
intrahash = {3cd605d75291fe2e1dd96525760e2c05},
issn = {0014-2999 (Print) 0014-2999 (Linking)},
journal = {Eur J Pharmacol},
keywords = {& Adrenergic Animals Assay Blotting, Cell Chain Cricetinae Cultured Cycle Down-Regulation Exotoxins/pharmacology Expression Gene Inhibitors/pharmacology Isoproterenol/antagonists Line Messenger/*analysis Northern Polymerase Protein RNA, Radioligand Rats Reaction Regulation Synthesis Tumor beta-Agonists/*pharmacology beta/*genetics inhibitors/pharmacology Receptor},
month = {Jul 16},
note = {Danner, S Lohse, M J Comparative Study Research Support, Non-U.S.
Gov't Netherlands European journal of pharmacology Eur J Pharmacol.
1997 Jul 16;331(1):73-8.},
number = 1,
pages = {73-8},
shorttitle = {Cell type-specific regulation of beta2-adrenoceptor mRNA by agonists},
timestamp = {2010-12-14T18:22:39.000+0100},
title = {Cell type-specific regulation of beta2-adrenoceptor mRNA by agonists},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9274932},
volume = 331,
year = 1997
}