The evaluation of the nanoparticles interactions with blood plasma
enables a first and important insight on the organization of the
adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe3O4@calix-Eu(TTA) nanoparticles
in human plasma. The difference in the surface chemistry of F3O4
functionalized with calixarene (+30 mV zeta-potential) and europium
(III) thenoyltrifluoroacetonate (Eu3+-TTA) complex (+7.4 mV
zeta-potential) affected the colloidal stability and hard corona
composition of the nanoparticles, which were monitored by SDS-PAGE gel
electrophoresis, differential centrifugal sedimentation analyses and
luminescence spectroscopy. Strikingly, after conjugation with Eu3+ TTA
complex, the magnetic nanoparticles show lower adsorption affinity to
proteins at higher blood plasma concentration. Moreover, the Eu3+
compound is featured with the narrow emission lines of D-5(0)-> F-7(J) (J=0-4) transitions, imparting additional characteristics to
bifunctional nanoparticles that were used as probes to evaluate the
protein corona formation.
%0 Journal Article
%1 WOS:000452048600002
%A Khan, Latif U
%A Petry, Romana
%A Paula, Amauri J
%A Knobel, Marcelo
%A Martinez, Diego Stefani T
%C POSTFACH 101161, 69451 WEINHEIM, GERMANY
%D 2018
%I WILEY-V C H VERLAG GMBH
%J CHEMNANOMAT
%K corona} europium; magnetite; nanoprobe; protein {bifunctional
%N 12
%P 1202-1208
%R 10.1002/cnma.201800358
%T Protein Corona Formation on Magnetic Nanoparticles Conjugated with
Luminescent Europium Complexes
%V 4
%X The evaluation of the nanoparticles interactions with blood plasma
enables a first and important insight on the organization of the
adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe3O4@calix-Eu(TTA) nanoparticles
in human plasma. The difference in the surface chemistry of F3O4
functionalized with calixarene (+30 mV zeta-potential) and europium
(III) thenoyltrifluoroacetonate (Eu3+-TTA) complex (+7.4 mV
zeta-potential) affected the colloidal stability and hard corona
composition of the nanoparticles, which were monitored by SDS-PAGE gel
electrophoresis, differential centrifugal sedimentation analyses and
luminescence spectroscopy. Strikingly, after conjugation with Eu3+ TTA
complex, the magnetic nanoparticles show lower adsorption affinity to
proteins at higher blood plasma concentration. Moreover, the Eu3+
compound is featured with the narrow emission lines of D-5(0)-> F-7(J) (J=0-4) transitions, imparting additional characteristics to
bifunctional nanoparticles that were used as probes to evaluate the
protein corona formation.
@article{WOS:000452048600002,
abstract = {The evaluation of the nanoparticles interactions with blood plasma
enables a first and important insight on the organization of the
adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe3O4@calix-Eu(TTA) nanoparticles
in human plasma. The difference in the surface chemistry of F3O4
functionalized with calixarene (+30 mV zeta-potential) and europium
(III) thenoyltrifluoroacetonate (Eu3+-TTA) complex (+7.4 mV
zeta-potential) affected the colloidal stability and hard corona
composition of the nanoparticles, which were monitored by SDS-PAGE gel
electrophoresis, differential centrifugal sedimentation analyses and
luminescence spectroscopy. Strikingly, after conjugation with Eu3+ TTA
complex, the magnetic nanoparticles show lower adsorption affinity to
proteins at higher blood plasma concentration. Moreover, the Eu3+
compound is featured with the narrow emission lines of D-5(0)-> F-7(J) (J=0-4) transitions, imparting additional characteristics to
bifunctional nanoparticles that were used as probes to evaluate the
protein corona formation.},
added-at = {2022-05-23T20:00:14.000+0200},
address = {POSTFACH 101161, 69451 WEINHEIM, GERMANY},
author = {Khan, Latif U and Petry, Romana and Paula, Amauri J and Knobel, Marcelo and Martinez, Diego Stefani T},
biburl = {https://www.bibsonomy.org/bibtex/247180e1b84867a96cbf221b00c8590c0/ppgfis_ufc_br},
doi = {10.1002/cnma.201800358},
interhash = {5627877a4d768dec3523316ed63c8f1e},
intrahash = {47180e1b84867a96cbf221b00c8590c0},
issn = {2199-692X},
journal = {CHEMNANOMAT},
keywords = {corona} europium; magnetite; nanoprobe; protein {bifunctional},
number = 12,
pages = {1202-1208},
publisher = {WILEY-V C H VERLAG GMBH},
pubstate = {published},
timestamp = {2022-05-23T20:00:14.000+0200},
title = {Protein Corona Formation on Magnetic Nanoparticles Conjugated with
Luminescent Europium Complexes},
tppubtype = {article},
volume = 4,
year = 2018
}