%0 Journal Article %1 dertinger2002gradients %A Dertinger, Stephan K. W. %A Jiang, Xingyu %A Li, Zhiying %A Murthy, Venkatesh N. %A Whitesides, George M. %D 2002 %J Proceedings of the National Academy of Sciences %K fabrication functionalization neurons phd todo %N 20 %P 12542--12547 %R 10.1073/pnas.192457199 %T Gradients of substrate-bound laminin orient axonal specification of neurons %U http://www.pnas.org/content/99/20/12542.abstract %V 99 %X Little is known about the influence of substrate-bound gradients on neuronal development, since it has been difficult to fabricate gradients over the distances typically required for biological studies (a few hundred micrometers). This article demonstrates a generally applicable technique for the fabrication of substrate-bound gradients of proteins with complex shapes, using laminar flows in microchannels. Gradients that range from pure laminin to pure BSA were formed in solution by using a network of microchannels, and these proteins were allowed to adsorb onto a homogeneous layer of poly-l-lysine. Rat hippocampal neurons were cultivated on these substrate-bound gradients. Analysis of optical images of these neurons showed that axon specification is oriented in the direction of increasing surface density of laminin. Linear gradients in laminin adsorbed from a gradient in solution having a slope of ∇laminin > about 0.06 μg (ml⋅μm)−1 (defined by dividing the change of concentration of laminin in solution over the distance of the gradient) orient axon specification, whereas those with ∇laminin < about 0.06 μg (ml⋅μm)−1 have no effect.

@article{dertinger2002gradients, abstract = {Little is known about the influence of substrate-bound gradients on neuronal development, since it has been difficult to fabricate gradients over the distances typically required for biological studies (a few hundred micrometers). This article demonstrates a generally applicable technique for the fabrication of substrate-bound gradients of proteins with complex shapes, using laminar flows in microchannels. Gradients that range from pure laminin to pure BSA were formed in solution by using a network of microchannels, and these proteins were allowed to adsorb onto a homogeneous layer of poly-l-lysine. Rat hippocampal neurons were cultivated on these substrate-bound gradients. Analysis of optical images of these neurons showed that axon specification is oriented in the direction of increasing surface density of laminin. Linear gradients in laminin adsorbed from a gradient in solution having a slope of ∇[laminin] > about 0.06 μg (ml⋅μm)−1 (defined by dividing the change of concentration of laminin in solution over the distance of the gradient) orient axon specification, whereas those with ∇[laminin] < about 0.06 μg (ml⋅μm)−1 have no effect.}, added-at = {2013-01-29T14:23:50.000+0100}, author = {Dertinger, Stephan K. W. and Jiang, Xingyu and Li, Zhiying and Murthy, Venkatesh N. and Whitesides, George M.}, biburl = {https://www.bibsonomy.org/bibtex/25921deff2f7d4e30c039e91180ba79ab/bkoch}, description = {Gradients of substrate-bound laminin orient axonal specification of neurons}, doi = {10.1073/pnas.192457199}, eprint = {http://www.pnas.org/content/99/20/12542.full.pdf+html}, interhash = {66cebf2a3d7a4b8bdb61075096dfad89}, intrahash = {5921deff2f7d4e30c039e91180ba79ab}, journal = {Proceedings of the National Academy of Sciences}, keywords = {fabrication functionalization neurons phd todo}, number = 20, pages = {12542--12547}, timestamp = {2013-01-29T14:23:59.000+0100}, title = {Gradients of substrate-bound laminin orient axonal specification of neurons}, url = {http://www.pnas.org/content/99/20/12542.abstract}, volume = 99, year = 2002 }