Little is known about the influence of substrate-bound gradients on neuronal development, since it has been difficult to fabricate gradients over the distances typically required for biological studies (a few hundred micrometers). This article demonstrates a generally applicable technique for the fabrication of substrate-bound gradients of proteins with complex shapes, using laminar flows in microchannels. Gradients that range from pure laminin to pure BSA were formed in solution by using a network of microchannels, and these proteins were allowed to adsorb onto a homogeneous layer of poly-l-lysine. Rat hippocampal neurons were cultivated on these substrate-bound gradients. Analysis of optical images of these neurons showed that axon specification is oriented in the direction of increasing surface density of laminin. Linear gradients in laminin adsorbed from a gradient in solution having a slope of ∇laminin > about 0.06 μg (ml⋅μm)−1 (defined by dividing the change of concentration of laminin in solution over the distance of the gradient) orient axon specification, whereas those with ∇laminin < about 0.06 μg (ml⋅μm)−1 have no effect.
Description
Gradients of substrate-bound laminin orient axonal specification of neurons
%0 Journal Article
%1 dertinger2002gradients
%A Dertinger, Stephan K. W.
%A Jiang, Xingyu
%A Li, Zhiying
%A Murthy, Venkatesh N.
%A Whitesides, George M.
%D 2002
%J Proceedings of the National Academy of Sciences
%K fabrication functionalization neurons phd todo
%N 20
%P 12542--12547
%R 10.1073/pnas.192457199
%T Gradients of substrate-bound laminin orient axonal specification of neurons
%U http://www.pnas.org/content/99/20/12542.abstract
%V 99
%X Little is known about the influence of substrate-bound gradients on neuronal development, since it has been difficult to fabricate gradients over the distances typically required for biological studies (a few hundred micrometers). This article demonstrates a generally applicable technique for the fabrication of substrate-bound gradients of proteins with complex shapes, using laminar flows in microchannels. Gradients that range from pure laminin to pure BSA were formed in solution by using a network of microchannels, and these proteins were allowed to adsorb onto a homogeneous layer of poly-l-lysine. Rat hippocampal neurons were cultivated on these substrate-bound gradients. Analysis of optical images of these neurons showed that axon specification is oriented in the direction of increasing surface density of laminin. Linear gradients in laminin adsorbed from a gradient in solution having a slope of ∇laminin > about 0.06 μg (ml⋅μm)−1 (defined by dividing the change of concentration of laminin in solution over the distance of the gradient) orient axon specification, whereas those with ∇laminin < about 0.06 μg (ml⋅μm)−1 have no effect.
@article{dertinger2002gradients,
abstract = {Little is known about the influence of substrate-bound gradients on neuronal development, since it has been difficult to fabricate gradients over the distances typically required for biological studies (a few hundred micrometers). This article demonstrates a generally applicable technique for the fabrication of substrate-bound gradients of proteins with complex shapes, using laminar flows in microchannels. Gradients that range from pure laminin to pure BSA were formed in solution by using a network of microchannels, and these proteins were allowed to adsorb onto a homogeneous layer of poly-l-lysine. Rat hippocampal neurons were cultivated on these substrate-bound gradients. Analysis of optical images of these neurons showed that axon specification is oriented in the direction of increasing surface density of laminin. Linear gradients in laminin adsorbed from a gradient in solution having a slope of ∇[laminin] > about 0.06 μg (ml⋅μm)−1 (defined by dividing the change of concentration of laminin in solution over the distance of the gradient) orient axon specification, whereas those with ∇[laminin] < about 0.06 μg (ml⋅μm)−1 have no effect.},
added-at = {2013-01-29T14:23:50.000+0100},
author = {Dertinger, Stephan K. W. and Jiang, Xingyu and Li, Zhiying and Murthy, Venkatesh N. and Whitesides, George M.},
biburl = {https://www.bibsonomy.org/bibtex/25921deff2f7d4e30c039e91180ba79ab/bkoch},
description = {Gradients of substrate-bound laminin orient axonal specification of neurons},
doi = {10.1073/pnas.192457199},
eprint = {http://www.pnas.org/content/99/20/12542.full.pdf+html},
interhash = {66cebf2a3d7a4b8bdb61075096dfad89},
intrahash = {5921deff2f7d4e30c039e91180ba79ab},
journal = {Proceedings of the National Academy of Sciences},
keywords = {fabrication functionalization neurons phd todo},
number = 20,
pages = {12542--12547},
timestamp = {2013-01-29T14:23:59.000+0100},
title = {Gradients of substrate-bound laminin orient axonal specification of neurons},
url = {http://www.pnas.org/content/99/20/12542.abstract},
volume = 99,
year = 2002
}