%0 Journal Article %1 Wen05 %A Wen, Jie %A Huang, Suming %A Rogers, Heather %A Dickinson, Liliane A %A Kohwi-Shigematsu, Terumi %A Noguchi, Constance Tom %D 2005 %J Blood %K Cell_Differentiation Chromatin Erythroid_Cells Gene_Expression_Regulation,_Developmental Globins Hematopoietic_Stem_Cells Hemin Humans K562_Cells Matrix_Attachment_Region_Binding_Proteins Multigene_Family Nuclear_Proteins Trans-Activators Transcription,_Genetic Transfection %N 8 %P 3330-9 %R 10.1182/blood-2004-08-2988 %T SATB1 family protein expressed during early erythroid differentiation modifies globin gene expression. %U http://dx.doi.org/10.1182/blood-2004-08-2988 %V 105 %X Special AT-rich binding protein 1 (SATB1) nuclear protein, expressed predominantly in T cells, regulates genes through targeting chromatin remodeling during T-cell maturation. Here we show SATB1 family protein induction during early human adult erythroid progenitor cell differentiation concomitant with epsilon-globin expression. Erythroid differentiation of human erythroleukemia K562 cells by hemin simultaneously increases gamma-globin and down-regulates SATB1 family protein and epsilon-globin gene expression. Chromatin immunoprecipitation using anti-SATB1 anti-body shows selective binding in vivo in the beta-globin cluster to the hypersensitive site 2 (HS2) in the locus control region (LCR) and to the epsilon-globin promoter. SATB1 overexpression increases epsilon-globin and decreases gamma-globin gene expression accompanied by histone hyperacetylation and hypomethylation in chromatin from the epsilon-globin promoter and HS2, and histone hypoacetylation and hypermethylation associated with the gamma-globin promoter. In K562 cells SATB1 family protein forms a complex with CREB-binding protein (CBP) important in transcriptional activation. In cotransfection experiments, increase in epsilon-promoter activity by SATB1 was amplified by CBP and blocked by E1A, a CBP inhibitor. Our results suggest that SATB1 can up-regulate the epsilon-globin gene by interaction with specific sites in the beta-globin cluster and imply that SATB1 family protein expressed in the erythroid progenitor cells may have a role in globin gene expression during early erythroid differentiation.

@article{Wen05, abstract = {Special AT-rich binding protein 1 (SATB1) nuclear protein, expressed predominantly in T cells, regulates genes through targeting chromatin remodeling during T-cell maturation. Here we show SATB1 family protein induction during early human adult erythroid progenitor cell differentiation concomitant with epsilon-globin expression. Erythroid differentiation of human erythroleukemia K562 cells by hemin simultaneously increases gamma-globin and down-regulates SATB1 family protein and epsilon-globin gene expression. Chromatin immunoprecipitation using anti-SATB1 anti-body shows selective binding in vivo in the beta-globin cluster to the hypersensitive site 2 (HS2) in the locus control region (LCR) and to the epsilon-globin promoter. SATB1 overexpression increases epsilon-globin and decreases gamma-globin gene expression accompanied by histone hyperacetylation and hypomethylation in chromatin from the epsilon-globin promoter and HS2, and histone hypoacetylation and hypermethylation associated with the gamma-globin promoter. In K562 cells SATB1 family protein forms a complex with CREB-binding protein (CBP) important in transcriptional activation. In cotransfection experiments, increase in epsilon-promoter activity by SATB1 was amplified by CBP and blocked by E1A, a CBP inhibitor. Our results suggest that SATB1 can up-regulate the epsilon-globin gene by interaction with specific sites in the beta-globin cluster and imply that SATB1 family protein expressed in the erythroid progenitor cells may have a role in globin gene expression during early erythroid differentiation.}, added-at = {2010-01-26T20:35:53.000+0100}, author = {Wen, Jie and Huang, Suming and Rogers, Heather and Dickinson, Liliane A and Kohwi-Shigematsu, Terumi and Noguchi, Constance Tom}, biburl = {https://www.bibsonomy.org/bibtex/26dbce417500dbc6322741be05759f819/denilw}, doi = {10.1182/blood-2004-08-2988}, file = {article:../papers/Transcription Factor Binding/SatB1/SATB1 family protein expressed during early erythroid differentiation promote breast tumour growth and Attachment Region-binding Domain of the Transcription.pdf:PDF}, institution = {National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.}, interhash = {ccb24664df5fa4f40ded7f1b3232754b}, intrahash = {6dbce417500dbc6322741be05759f819}, journal = {Blood}, keywords = {Cell_Differentiation Chromatin Erythroid_Cells Gene_Expression_Regulation,_Developmental Globins Hematopoietic_Stem_Cells Hemin Humans K562_Cells Matrix_Attachment_Region_Binding_Proteins Multigene_Family Nuclear_Proteins Trans-Activators Transcription,_Genetic Transfection}, month = Apr, number = 8, owner = {denilw}, pages = {3330-9}, pii = {2004-08-2988}, pmid = {15618465}, timestamp = {2010-01-26T20:36:05.000+0100}, title = {SATB1 family protein expressed during early erythroid differentiation modifies globin gene expression.}, url = {http://dx.doi.org/10.1182/blood-2004-08-2988}, volume = 105, year = 2005 }