Sphingosylphosphocholine is a naturally occurring lipid mediator
in blood plasma: a possible role in regulating cardiac function via
sphingolipid receptors
K. Liliom, G. Sun, M. Bunemann, T. Virag, N. Nusser, D. Baker, D. Wang, M. Fabian, B. Brandts, K. Bender, A. Eickel, K. Malik, D. Miller, D. Desiderio, G. Tigyi, and L. Pott. Biochem J, 355 (Pt 1):
189-97(April 2001)Liliom, K Sun, G Bunemann, M Virag, T Nusser, N Baker, D L Wang,
D A Fabian, M J Brandts, B Bender, K Eickel, A Malik, K U Miller,
D D Desiderio, D M Tigyi, G Pott, L D RR 10522/RR/NCRR NIH HHS/United
States HL61649/HL/NHLBI NIH HHS/United States Research Support, Non-U.S.
Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support,
U.S. Gov't, P.H.S. England The Biochemical journal Biochem J. 2001
Apr 1;355(Pt 1):189-97..
Abstract
Blood plasma and serum contain factors that activate inwardly rectifying
GIRK1/GIRK4 K+ channels in atrial myocytes via one or more non-atropine-sensitive
receptors coupled to pertussis-toxin-sensitive G-proteins. This channel
is also the target of muscarinic M(2) receptors activated by the
physiological release of acetylcholine from parasympathetic nerve
endings. By using a combination of HPLC and TLC techniques with matrix-assisted
laser desorption ionization-time-of-flight MS, we purified and identified
sphingosine 1-phosphate (SPP) and sphingosylphosphocholine (SPC)
as the plasma and serum factors responsible for activating the inwardly
rectifying K+ channel (I(K)). With the use of MS the concentration
of SPC was estimated at 50 nM in plasma and 130 nM in serum; those
concentrations exceeded the 1.5 nM EC(50) measured in guinea-pig
atrial myocytes. With the use of reverse-transcriptase-mediated PCR
and/or Western blot analysis, we detected Edg1, Edg3, Edg5 and Edg8
as well as OGR1 sphingolipid receptor transcripts and/or proteins.
In perfused guinea-pig hearts, SPC exerted a negative chronotropic
effect with a threshold concentration of 1 microM. SPC was completely
removed after perfusion through the coronary circulation at a concentration
of 10 microM. On the basis of their constitutive presence in plasma,
the expression of specific receptors, and a mechanism of ligand inactivation,
we propose that SPP and SPC might have a physiologically relevant
role in the regulation of the heart.
Sphingosylphosphocholine is a naturally occurring lipid mediator in
blood plasma: a possible role in regulating cardiac function via
sphingolipid receptors
Liliom, K Sun, G Bunemann, M Virag, T Nusser, N Baker, D L Wang,
D A Fabian, M J Brandts, B Bender, K Eickel, A Malik, K U Miller,
D D Desiderio, D M Tigyi, G Pott, L D RR 10522/RR/NCRR NIH HHS/United
States HL61649/HL/NHLBI NIH HHS/United States Research Support, Non-U.S.
Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support,
U.S. Gov't, P.H.S. England The Biochemical journal Biochem J. 2001
Apr 1;355(Pt 1):189-97.
%0 Journal Article
%1 Liliom2001
%A Liliom, K.
%A Sun, G.
%A Bunemann, M.
%A Virag, T.
%A Nusser, N.
%A Baker, D. L.
%A Wang, D. A.
%A Fabian, M. J.
%A Brandts, B.
%A Bender, K.
%A Eickel, A.
%A Malik, K. U.
%A Miller, D. D.
%A Desiderio, D. M.
%A Tigyi, G.
%A Pott, L.
%D 2001
%J Biochem J
%K & Animals Atria/metabolism Blotting, Carrier Chain Desorption-Ionization Heart Heart/*physiology Laser Mass, Matrix-Assisted Phosphorylcholine/*analogs Polymerase Precipitin Proteins/metabolism/*physiology Rabbits Rats Reaction Reverse Spectrometry, Sphingolipids/*metabolism Sphingosine/*analogs Tests Transcriptase Western derivatives/*blood
%N Pt 1
%P 189-97
%T Sphingosylphosphocholine is a naturally occurring lipid mediator
in blood plasma: a possible role in regulating cardiac function via
sphingolipid receptors
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11256963
%V 355
%X Blood plasma and serum contain factors that activate inwardly rectifying
GIRK1/GIRK4 K+ channels in atrial myocytes via one or more non-atropine-sensitive
receptors coupled to pertussis-toxin-sensitive G-proteins. This channel
is also the target of muscarinic M(2) receptors activated by the
physiological release of acetylcholine from parasympathetic nerve
endings. By using a combination of HPLC and TLC techniques with matrix-assisted
laser desorption ionization-time-of-flight MS, we purified and identified
sphingosine 1-phosphate (SPP) and sphingosylphosphocholine (SPC)
as the plasma and serum factors responsible for activating the inwardly
rectifying K+ channel (I(K)). With the use of MS the concentration
of SPC was estimated at 50 nM in plasma and 130 nM in serum; those
concentrations exceeded the 1.5 nM EC(50) measured in guinea-pig
atrial myocytes. With the use of reverse-transcriptase-mediated PCR
and/or Western blot analysis, we detected Edg1, Edg3, Edg5 and Edg8
as well as OGR1 sphingolipid receptor transcripts and/or proteins.
In perfused guinea-pig hearts, SPC exerted a negative chronotropic
effect with a threshold concentration of 1 microM. SPC was completely
removed after perfusion through the coronary circulation at a concentration
of 10 microM. On the basis of their constitutive presence in plasma,
the expression of specific receptors, and a mechanism of ligand inactivation,
we propose that SPP and SPC might have a physiologically relevant
role in the regulation of the heart.
@article{Liliom2001,
abstract = {Blood plasma and serum contain factors that activate inwardly rectifying
GIRK1/GIRK4 K+ channels in atrial myocytes via one or more non-atropine-sensitive
receptors coupled to pertussis-toxin-sensitive G-proteins. This channel
is also the target of muscarinic M(2) receptors activated by the
physiological release of acetylcholine from parasympathetic nerve
endings. By using a combination of HPLC and TLC techniques with matrix-assisted
laser desorption ionization-time-of-flight MS, we purified and identified
sphingosine 1-phosphate (SPP) and sphingosylphosphocholine (SPC)
as the plasma and serum factors responsible for activating the inwardly
rectifying K+ channel (I(K)). With the use of MS the concentration
of SPC was estimated at 50 nM in plasma and 130 nM in serum; those
concentrations exceeded the 1.5 nM EC(50) measured in guinea-pig
atrial myocytes. With the use of reverse-transcriptase-mediated PCR
and/or Western blot analysis, we detected Edg1, Edg3, Edg5 and Edg8
as well as OGR1 sphingolipid receptor transcripts and/or proteins.
In perfused guinea-pig hearts, SPC exerted a negative chronotropic
effect with a threshold concentration of 1 microM. SPC was completely
removed after perfusion through the coronary circulation at a concentration
of 10 microM. On the basis of their constitutive presence in plasma,
the expression of specific receptors, and a mechanism of ligand inactivation,
we propose that SPP and SPC might have a physiologically relevant
role in the regulation of the heart.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Liliom, K. and Sun, G. and Bunemann, M. and Virag, T. and Nusser, N. and Baker, D. L. and Wang, D. A. and Fabian, M. J. and Brandts, B. and Bender, K. and Eickel, A. and Malik, K. U. and Miller, D. D. and Desiderio, D. M. and Tigyi, G. and Pott, L.},
biburl = {https://www.bibsonomy.org/bibtex/285cf0ca5ab60d7f5e25b9a2a7874ad96/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {1782645a669d8b4d29709620f629a99a},
intrahash = {85cf0ca5ab60d7f5e25b9a2a7874ad96},
issn = {0264-6021 (Print) 0264-6021 (Linking)},
journal = {Biochem J},
keywords = {& Animals Atria/metabolism Blotting, Carrier Chain Desorption-Ionization Heart Heart/*physiology Laser Mass, Matrix-Assisted Phosphorylcholine/*analogs Polymerase Precipitin Proteins/metabolism/*physiology Rabbits Rats Reaction Reverse Spectrometry, Sphingolipids/*metabolism Sphingosine/*analogs Tests Transcriptase Western derivatives/*blood},
month = {Apr 1},
note = {Liliom, K Sun, G Bunemann, M Virag, T Nusser, N Baker, D L Wang,
D A Fabian, M J Brandts, B Bender, K Eickel, A Malik, K U Miller,
D D Desiderio, D M Tigyi, G Pott, L D RR 10522/RR/NCRR NIH HHS/United
States HL61649/HL/NHLBI NIH HHS/United States Research Support, Non-U.S.
Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support,
U.S. Gov't, P.H.S. England The Biochemical journal Biochem J. 2001
Apr 1;355(Pt 1):189-97.},
number = {Pt 1},
pages = {189-97},
shorttitle = {Sphingosylphosphocholine is a naturally occurring lipid mediator in
blood plasma: a possible role in regulating cardiac function via
sphingolipid receptors},
timestamp = {2010-12-14T18:12:20.000+0100},
title = {Sphingosylphosphocholine is a naturally occurring lipid mediator
in blood plasma: a possible role in regulating cardiac function via
sphingolipid receptors},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11256963},
volume = 355,
year = 2001
}