Angiogenesis and vasculogenesis are regulated in large part by several different growth factors and their associated receptor tyrosine kinases (RTKs). Foremost among these is the vascular endothelial growth factor (VEGF) family including VEGF receptor (VEGFR)-2 and -1. VEGFR ligand binding and biological activity are regulated at many levels, one of which is by a soluble, circulating form of VEGFR-1 (sVEGFR-1). This sVEGFR-1 can act as a competitive inhibitor of its ligand, serve as a possible biomarker, and play important roles in cancer and other diseases such as preeclampsia. Recombinant forms of sVEGFR-2 have been shown to have antiangiogenic activity, but a naturally occurring sVEGFR-2 has not been described previously. Here, we report such an entity. Having a molecular weight of ~160 kDa, sVEGFR-2 can be detected in mouse and human plasma with several different monoclonal and polyclonal anti-VEGFR-2 antibodies using both ELISA and immunoprecipitation techniques. In vitro studie
%0 Journal Article
%1 Ebos.2004
%A Ebos, John M. L.
%A Bocci, Guido
%A Man, Shan
%A Thorpe, Philip E.
%A Hicklin, Daniel J.
%A Zhou, Danielle
%A Jia, Xiaohong
%A Kerbel, Robert S.
%D 2004
%J Mol Cancer Res
%K Antibodies Binding Cells Endothelial Human Immunoprecipitation Molecular Tyrosine Weight cells protein
%N 6
%P 315-326
%T A Naturally Occurring Soluble Form of Vascular Endothelial Growth Factor Receptor 2 Detected in Mouse and Human Plasma
%U http://mcr.aacrjournals.org/cgi/content/abstract/2/6/315
%V 2
%X Angiogenesis and vasculogenesis are regulated in large part by several different growth factors and their associated receptor tyrosine kinases (RTKs). Foremost among these is the vascular endothelial growth factor (VEGF) family including VEGF receptor (VEGFR)-2 and -1. VEGFR ligand binding and biological activity are regulated at many levels, one of which is by a soluble, circulating form of VEGFR-1 (sVEGFR-1). This sVEGFR-1 can act as a competitive inhibitor of its ligand, serve as a possible biomarker, and play important roles in cancer and other diseases such as preeclampsia. Recombinant forms of sVEGFR-2 have been shown to have antiangiogenic activity, but a naturally occurring sVEGFR-2 has not been described previously. Here, we report such an entity. Having a molecular weight of ~160 kDa, sVEGFR-2 can be detected in mouse and human plasma with several different monoclonal and polyclonal anti-VEGFR-2 antibodies using both ELISA and immunoprecipitation techniques. In vitro studie
@article{Ebos.2004,
abstract = {Angiogenesis and vasculogenesis are regulated in large part by several different growth factors and their associated receptor tyrosine kinases (RTKs). Foremost among these is the vascular endothelial growth factor (VEGF) family including VEGF receptor (VEGFR)-2 and -1. VEGFR ligand binding and biological activity are regulated at many levels, one of which is by a soluble, circulating form of VEGFR-1 (sVEGFR-1). This sVEGFR-1 can act as a competitive inhibitor of its ligand, serve as a possible biomarker, and play important roles in cancer and other diseases such as preeclampsia. Recombinant forms of sVEGFR-2 have been shown to have antiangiogenic activity, but a naturally occurring sVEGFR-2 has not been described previously. Here, we report such an entity. Having a molecular weight of [~]160 kDa, sVEGFR-2 can be detected in mouse and human plasma with several different monoclonal and polyclonal anti-VEGFR-2 antibodies using both ELISA and immunoprecipitation techniques. In vitro studie},
added-at = {2010-02-05T11:28:39.000+0100},
author = {Ebos, John M. L. and Bocci, Guido and Man, Shan and Thorpe, Philip E. and Hicklin, Daniel J. and Zhou, Danielle and Jia, Xiaohong and Kerbel, Robert S.},
biburl = {https://www.bibsonomy.org/bibtex/2d0dc4b810c887a06c251a5eed3208794/kanefendt},
interhash = {9e37cc825927b4219f2116ad88d188a1},
intrahash = {d0dc4b810c887a06c251a5eed3208794},
journal = {Mol Cancer Res},
keywords = {Antibodies Binding Cells Endothelial Human Immunoprecipitation Molecular Tyrosine Weight cells protein},
number = 6,
pages = {315-326},
timestamp = {2010-02-05T11:28:42.000+0100},
title = {A Naturally Occurring Soluble Form of Vascular Endothelial Growth Factor Receptor 2 Detected in Mouse and Human Plasma},
url = {http://mcr.aacrjournals.org/cgi/content/abstract/2/6/315},
volume = 2,
year = 2004
}