A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples
%0 Journal Article
%1 Chomczynski.1987
%A Chomczynski, P.
%A Sacchi, N.
%D 1987
%J Anal.Biochem.
%K & Animal Animals Cell Chloroform Concentration Glands Guanidine Guanidines Humans Hydrogen-Ion Line Mammary Phenol Phenols RNA Rats Solutions analysis cells isolation purification
%N 1
%P 156-159
%T Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
%U PM:2440339
%V 162
%X A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples
@article{Chomczynski.1987,
abstract = {A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples},
added-at = {2010-02-05T11:28:39.000+0100},
author = {Chomczynski, P. and Sacchi, N.},
biburl = {https://www.bibsonomy.org/bibtex/2e0c24eb6bc035e5246ea5789b096d844/kanefendt},
interhash = {085d1c746c723e11d4872bae3265cada},
intrahash = {e0c24eb6bc035e5246ea5789b096d844},
journal = {Anal.Biochem.},
keywords = {& Animal Animals Cell Chloroform Concentration Glands Guanidine Guanidines Humans Hydrogen-Ion Line Mammary Phenol Phenols RNA Rats Solutions analysis cells isolation purification},
number = 1,
pages = {156-159},
timestamp = {2010-02-05T11:28:44.000+0100},
title = {Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction},
url = {PM:2440339},
volume = 162,
year = 1987
}