A family of outer membrane lipoproteins of Neisseria meningitidis, LP2086, has been shown to induce serum bactericidal activity against a broad variety of meningococcal strains. Two sub-families of serologically distinct LP2086 proteins (A and B) have been identified. In the present study, we have shown that polyclonal anti-serum against rLP2086 is protective in vivo in an infant rat passive-protection model. Additionally, the LP2086 protein is displayed on the surface of 91\% meningococcal strains as measured in a whole cell ELISA using polyclonal anti-sera raised against these proteins. We also demonstrate based on the reactivity of anti-rLP2086 antibody with recombinantly expressed C- and N-terminal fragments of rLP2086 in a Western blot assay that the C-terminal fragment of LP2086 dictates sub-family specificity and the N-terminal fragment determines the family specificity. A formulation containing family A and B of LP2086 potentially would provide broad protection against a majority of Neisseria meningitidis strains.
%0 Journal Article
%1 pillai_outer_2005
%A Pillai, Subramonia
%A Howell, Alan
%A Alexander, Kristin
%A Bentley, B Erin
%A Jiang, Han-Qian
%A Ambrose, Karita
%A Zhu, Duzhang
%A Zlotnick, Gary
%D 2005
%J Vaccine
%K Animals, Antibodies, B, Bacterial Bacterial, Humans, Infections, Membrane Meningococcal Mice, Neisseria Outer Proteins, Rats, Serogroup Synthetic Vaccines, meningitidis, {Sprague-Dawley,}
%N 17-18
%P 2206--9
%R S0264-410X(05)00061-7
%T Outer membrane protein (OMP) based vaccine for Neisseria meningitidis serogroup B
%V 23
%X A family of outer membrane lipoproteins of Neisseria meningitidis, LP2086, has been shown to induce serum bactericidal activity against a broad variety of meningococcal strains. Two sub-families of serologically distinct LP2086 proteins (A and B) have been identified. In the present study, we have shown that polyclonal anti-serum against rLP2086 is protective in vivo in an infant rat passive-protection model. Additionally, the LP2086 protein is displayed on the surface of 91\% meningococcal strains as measured in a whole cell ELISA using polyclonal anti-sera raised against these proteins. We also demonstrate based on the reactivity of anti-rLP2086 antibody with recombinantly expressed C- and N-terminal fragments of rLP2086 in a Western blot assay that the C-terminal fragment of LP2086 dictates sub-family specificity and the N-terminal fragment determines the family specificity. A formulation containing family A and B of LP2086 potentially would provide broad protection against a majority of Neisseria meningitidis strains.
@article{pillai_outer_2005,
abstract = {A family of outer membrane lipoproteins of Neisseria meningitidis, {LP2086,} has been shown to induce serum bactericidal activity against a broad variety of meningococcal strains. Two sub-families of serologically distinct {LP2086} proteins {(A} and B) have been identified. In the present study, we have shown that polyclonal anti-serum against {rLP2086} is protective in vivo in an infant rat passive-protection model. Additionally, the {LP2086} protein is displayed on the surface of 91\% meningococcal strains as measured in a whole cell {ELISA} using polyclonal anti-sera raised against these proteins. We also demonstrate based on the reactivity of {anti-rLP2086} antibody with recombinantly expressed C- and N-terminal fragments of {rLP2086} in a Western blot assay that the C-terminal fragment of {LP2086} dictates sub-family specificity and the N-terminal fragment determines the family specificity. A formulation containing family A and B of {LP2086} potentially would provide broad protection against a majority of Neisseria meningitidis strains.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Pillai, Subramonia and Howell, Alan and Alexander, Kristin and Bentley, B Erin and Jiang, {Han-Qian} and Ambrose, Karita and Zhu, Duzhang and Zlotnick, Gary},
biburl = {https://www.bibsonomy.org/bibtex/2fc2eef7b1337398cd489bda37881ce0c/jelias},
doi = {S0264-410X(05)00061-7},
interhash = {a611de6d33a03b0365f0197c43a9ba6e},
intrahash = {fc2eef7b1337398cd489bda37881ce0c},
issn = {{0264-410X}},
journal = {Vaccine},
keywords = {Animals, Antibodies, B, Bacterial Bacterial, Humans, Infections, Membrane Meningococcal Mice, Neisseria Outer Proteins, Rats, Serogroup Synthetic Vaccines, meningitidis, {Sprague-Dawley,}},
month = mar,
note = {{PMID:} 15755596},
number = {17-18},
pages = {2206--9},
timestamp = {2011-03-11T10:06:33.000+0100},
title = {Outer membrane protein {(OMP)} based vaccine for Neisseria meningitidis serogroup B},
volume = 23,
year = 2005
}