%0 Journal Article %1 WOS:000232799200032 %A de Farias, PMA %A Santos, BS %A de Menezes, FD %A Ferreira, RD %A Barias-Castro, ML %A Castro, V %A Lima, PRM %A Fontes, A %A Cesar, CL %C 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA %D 2005 %I SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS %J JOURNAL OF BIOMEDICAL OPTICS %K dots} materials; optical optics; quantum {biomedical %N 4 %R 10.1117/1.1993257 %T Investigation of red blood cell antigens with highly fluorescent and stable semiconductor quantum dots %V 10 %X We report a new methodology for red blood cell antigen expression determination by a simple labeling procedure employing luminescent semiconductor quantum dots. Highly luminescent and stable core shell cadmium sulfide/cadmium hydroxide colloidal particles are obtained, with a predominant size of 9 nm. The core-shell quantum dots are functionalized with glutaraldehyde and conjugated to a monoclonal anti-A antibody to target antigen-A in red blood cell membranes. Erythrocyte samples of blood groups A(+), A(2)(+), and O+ are used for this purpose. Confocal microscopy images show that after 30 min of conjugation time, type A(+) and A(2)(+) erythrocytes present bright emission, whereas the O+ group cells show no emission. Fluorescence intensity maps show different antigen expressions for the distinct erythrocyte types. The results obtained strongly suggest that this simple labeling procedure may be employed as an efficient tool to investigate quantitatively the distribution and expression of antigens in red blood cell membranes. (C) 2005 Society of Photo-Optical Instrumentation Engineers.

@article{WOS:000232799200032, abstract = {We report a new methodology for red blood cell antigen expression determination by a simple labeling procedure employing luminescent semiconductor quantum dots. Highly luminescent and stable core shell cadmium sulfide/cadmium hydroxide colloidal particles are obtained, with a predominant size of 9 nm. The core-shell quantum dots are functionalized with glutaraldehyde and conjugated to a monoclonal anti-A antibody to target antigen-A in red blood cell membranes. Erythrocyte samples of blood groups A(+), A(2)(+), and O+ are used for this purpose. Confocal microscopy images show that after 30 min of conjugation time, type A(+) and A(2)(+) erythrocytes present bright emission, whereas the O+ group cells show no emission. Fluorescence intensity maps show different antigen expressions for the distinct erythrocyte types. The results obtained strongly suggest that this simple labeling procedure may be employed as an efficient tool to investigate quantitatively the distribution and expression of antigens in red blood cell membranes. (C) 2005 Society of Photo-Optical Instrumentation Engineers.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA}, author = {de Farias, PMA and Santos, BS and de Menezes, FD and Ferreira, RD and Barias-Castro, ML and Castro, V and Lima, PRM and Fontes, A and Cesar, CL}, biburl = {https://www.bibsonomy.org/bibtex/2832ab8d02d34c43eb9926eec6beadf81/ppgfis_ufc_br}, doi = {10.1117/1.1993257}, interhash = {309644d4919f991049ba2d411c193e90}, intrahash = {832ab8d02d34c43eb9926eec6beadf81}, issn = {1083-3668}, journal = {JOURNAL OF BIOMEDICAL OPTICS}, keywords = {dots} materials; optical optics; quantum {biomedical}, number = 4, publisher = {SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Investigation of red blood cell antigens with highly fluorescent and stable semiconductor quantum dots}, tppubtype = {article}, volume = 10, year = 2005 }