%0 Journal Article %1 RN1130 %A Sano, T. %A Kutsuna, N. %A Becker, D. %A Hedrich, R. %A Hasezawa, S. %D 2009 %J Plant Journal %K cell division myOwn %N 1 %P 55-64 %R 10.1111/j.1365-313X.2008.03672.x %T Outward-rectifying K channel activities regulate cell elongation and cell division of tobacco BY-2 cells %U /brokenurl#<Go to ISI>://WOS:000261962700005 %V 57 %X Potassium ions (K+) are required for plant growth and development, including cell division and cell elongation/expansion, which are mediated by the K+ transport system. In this study, we investigated the role of K+ in cell division using tobacco BY-2 protoplast cultures. Gene expression analysis revealed induction of the Shaker-like outward K+ channel gene, NTORK1, under cell-division conditions, whereas the inward K+ channel genes NKT1 and NtKC1 were induced under both cell-elongation and cell-division conditions. Repression of NTORK1 gene expression by expression of its antisense construct repressed cell division but accelerated cell elongation even under conditions promoting cell division. A decrease in the K+ content of cells and cellular osmotic pressure in dividing cells suggested that an increase in cell osmotic pressure by K+ uptake is not required for cell division. In contrast, K+ depletion, which reduced cell-division activity, decreased cytoplasmic pH as monitored using a fluorescent pH indicator, SNARF-1. Application of K+ or the cytoplasmic alkalizing reagent (NH4)(2)SO4 increased cytoplasmic pH and suppressed the reduction in cell-division activity. These results suggest that the K+ taken up into cells is used to regulate cytoplasmic pH during cell division.

@article{RN1130, abstract = {Potassium ions (K+) are required for plant growth and development, including cell division and cell elongation/expansion, which are mediated by the K+ transport system. In this study, we investigated the role of K+ in cell division using tobacco BY-2 protoplast cultures. Gene expression analysis revealed induction of the Shaker-like outward K+ channel gene, NTORK1, under cell-division conditions, whereas the inward K+ channel genes NKT1 and NtKC1 were induced under both cell-elongation and cell-division conditions. Repression of NTORK1 gene expression by expression of its antisense construct repressed cell division but accelerated cell elongation even under conditions promoting cell division. A decrease in the K+ content of cells and cellular osmotic pressure in dividing cells suggested that an increase in cell osmotic pressure by K+ uptake is not required for cell division. In contrast, K+ depletion, which reduced cell-division activity, decreased cytoplasmic pH as monitored using a fluorescent pH indicator, SNARF-1. Application of K+ or the cytoplasmic alkalizing reagent (NH4)(2)SO4 increased cytoplasmic pH and suppressed the reduction in cell-division activity. These results suggest that the K+ taken up into cells is used to regulate cytoplasmic pH during cell division.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Sano, T. and Kutsuna, N. and Becker, D. and Hedrich, R. and Hasezawa, S.}, biburl = {https://www.bibsonomy.org/bibtex/2047f42ee0b2fbba6c316ce27566d15a3/rainerhedrich_2}, doi = {10.1111/j.1365-313X.2008.03672.x}, interhash = {58e9e1aadfd70ccd4f3aecd8d70ff319}, intrahash = {047f42ee0b2fbba6c316ce27566d15a3}, issn = {0960-7412}, journal = {Plant Journal}, keywords = {cell division myOwn}, note = {387oy Times Cited:27 Cited References Count:52}, number = 1, pages = {55-64}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Outward-rectifying K channel activities regulate cell elongation and cell division of tobacco BY-2 cells}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000261962700005}, volume = 57, year = 2009 }