%0 Journal Article %1 Roh2008 %A Roh, Tae-Young %A Zhao, Keji %D 2008 %J Methods Mol Biol %K Chromatin,_genetics Chromatin_Immunoprecipitation,_methods Chromosome_Mapping,_methods Chromosomes,_Fungal,_genetics Chromosomes,_Human,_Pair_12,_genetics Cloning,_Molecular DNA_Polymerase_I DNA_Primers,_genetics Deoxyribonucleases,_Type_II_Site-Specific Epigenesis,_Genetic Gene_Expression_Profiling,_methods Genome,_Fungal Genome,_Human Genomics,_methods Humans Polymerase_Chain_Reaction Saccharomyces_cerevisiae,_genetics Sequence_Analysis,_DNA %P 95-108 %T High-resolution, genome-wide mapping of chromatin modifications by GMAT. %V 387 %X One major postgenomic challenge is to characterize the epigenomes that control genome functions. The epigenomes are mainly defined by the specific association of nonhistone proteins with chromatin and the covalent modifications of chromatin, including DNA methylation and posttranslational histone modifications. The in vivo protein-binding and chromatin-modification patterns can be revealed by the chromatin immunoprecipitation assay (ChIP). By combining the ChIP assays and the serial analysis of gene expression (SAGE) protocols, we have developed an unbiased and high-resolution genome-wide mapping technique (GMAT) to determine the genome-wide protein-targeting and chromatin-modification patterns. GMAT has been successfully applied to mapping the target sites of the histone acetyltransferase, Gcn5p, in yeast and to the discovery of the histone acetylation islands as an epigenetic mark for functional regulatory elements in the human genome.

@article{Roh2008, abstract = {One major postgenomic challenge is to characterize the epigenomes that control genome functions. The epigenomes are mainly defined by the specific association of nonhistone proteins with chromatin and the covalent modifications of chromatin, including DNA methylation and posttranslational histone modifications. The in vivo protein-binding and chromatin-modification patterns can be revealed by the chromatin immunoprecipitation assay (ChIP). By combining the ChIP assays and the serial analysis of gene expression (SAGE) protocols, we have developed an unbiased and high-resolution genome-wide mapping technique (GMAT) to determine the genome-wide protein-targeting and chromatin-modification patterns. GMAT has been successfully applied to mapping the target sites of the histone acetyltransferase, Gcn5p, in yeast and to the discovery of the histone acetylation islands as an epigenetic mark for functional regulatory elements in the human genome.}, added-at = {2010-01-26T20:35:53.000+0100}, author = {Roh, Tae-Young and Zhao, Keji}, biburl = {https://www.bibsonomy.org/bibtex/2519321177bf9f3ff63be07d982c78c4c/denilw}, institution = {National Institute of Health, Bethesda, MD, USA.}, interhash = {5d7e75602c662776b885d699624fa976}, intrahash = {519321177bf9f3ff63be07d982c78c4c}, journal = {Methods Mol Biol}, keywords = {Chromatin,_genetics Chromatin_Immunoprecipitation,_methods Chromosome_Mapping,_methods Chromosomes,_Fungal,_genetics Chromosomes,_Human,_Pair_12,_genetics Cloning,_Molecular DNA_Polymerase_I DNA_Primers,_genetics Deoxyribonucleases,_Type_II_Site-Specific Epigenesis,_Genetic Gene_Expression_Profiling,_methods Genome,_Fungal Genome,_Human Genomics,_methods Humans Polymerase_Chain_Reaction Saccharomyces_cerevisiae,_genetics Sequence_Analysis,_DNA}, owner = {denilw}, pages = {95-108}, pii = {1-59745-454-0:95}, pmid = {18287625}, timestamp = {2010-01-26T20:36:02.000+0100}, title = {High-resolution, genome-wide mapping of chromatin modifications by GMAT.}, volume = 387, year = 2008 }