Abstract
Two simple, inexpensive media containing gonococcal agar-base, supplemented with 5\% Fildes' extract and either chocolated or unchocolated horse blood (GC-FHBC or GC-FHB) were compared with the standard gonococcal agar-based (GC-HgS) and Mueller-Hinton agar-based media (MH-HB) normally used for primary isolation of Haemophilus ducreyi from presumptive chancroid lesions. Overall, Haemophilus ducreyi was recovered from 162 of 178 (91\%) samples from primary chancroid lesions. As a single isolation medium GC-HgS proved the most sensitive with an isolation rate of 80\% followed by GC-FHB (75\%), MH-HB and GC-FHC (both 71\%). Use of a combination of GC-HgS and MH-HB resulted in isolation of Haemophilus ducreyi in 160 of 178 cases (90\%). Since GC-FHB is approximately one-quarter the cost of the combination and half the cost of GC-HgS or MH-HB alone, this medium could prove suitable for diagnostic purposes in developing countries where chancroid is endemic.
- africa
- agar,
- bacteriological
- chancroid,
- colony
- count,
- countries,
- culture
- developing
- ducreyi,
- haemophilus
- humans,
- male,
- media,
- microbial,
- south
- techniques,
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