%0 Journal Article %1 dangor_simple_1992 %A Dangor, Y %A Miller, S D %A Koornhof, H J %A Ballard, R C %D 1992 %J European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology %K Africa Agar, Bacteriological Chancroid, Colony Count, Countries, Culture Developing Haemophilus Humans, Male, Media, Microbial, South Techniques, ducreyi, %N 10 %P 930--934 %T A simple medium for the primary isolation of Haemophilus ducreyi %U http://www.ncbi.nlm.nih.gov/pubmed/1486890 %V 11 %X Two simple, inexpensive media containing gonococcal agar-base, supplemented with 5\% Fildes' extract and either chocolated or unchocolated horse blood (GC-FHBC or GC-FHB) were compared with the standard gonococcal agar-based (GC-HgS) and Mueller-Hinton agar-based media (MH-HB) normally used for primary isolation of Haemophilus ducreyi from presumptive chancroid lesions. Overall, Haemophilus ducreyi was recovered from 162 of 178 (91\%) samples from primary chancroid lesions. As a single isolation medium GC-HgS proved the most sensitive with an isolation rate of 80\% followed by GC-FHB (75\%), MH-HB and GC-FHC (both 71\%). Use of a combination of GC-HgS and MH-HB resulted in isolation of Haemophilus ducreyi in 160 of 178 cases (90\%). Since GC-FHB is approximately one-quarter the cost of the combination and half the cost of GC-HgS or MH-HB alone, this medium could prove suitable for diagnostic purposes in developing countries where chancroid is endemic.

@article{dangor_simple_1992, abstract = {Two simple, inexpensive media containing gonococcal agar-base, supplemented with 5\% Fildes' extract and either chocolated or unchocolated horse blood {(GC-FHBC} or {GC-FHB)} were compared with the standard gonococcal agar-based {(GC-HgS)} and {Mueller-Hinton} agar-based media {(MH-HB)} normally used for primary isolation of Haemophilus ducreyi from presumptive chancroid lesions. Overall, Haemophilus ducreyi was recovered from 162 of 178 (91\%) samples from primary chancroid lesions. As a single isolation medium {GC-HgS} proved the most sensitive with an isolation rate of 80\% followed by {GC-FHB} (75\%), {MH-HB} and {GC-FHC} (both 71\%). Use of a combination of {GC-HgS} and {MH-HB} resulted in isolation of Haemophilus ducreyi in 160 of 178 cases (90\%). Since {GC-FHB} is approximately one-quarter the cost of the combination and half the cost of {GC-HgS} or {MH-HB} alone, this medium could prove suitable for diagnostic purposes in developing countries where chancroid is endemic.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Dangor, Y and Miller, S D and Koornhof, H J and Ballard, R C}, biburl = {https://www.bibsonomy.org/bibtex/21fd553541e0158c2c6e42499368e9483/jelias}, interhash = {600f02f0cf41998fffbfa18bbb98ed72}, intrahash = {1fd553541e0158c2c6e42499368e9483}, issn = {0934-9723}, journal = {European Journal of Clinical Microbiology \& Infectious Diseases: Official Publication of the European Society of Clinical Microbiology}, keywords = {Africa Agar, Bacteriological Chancroid, Colony Count, Countries, Culture Developing Haemophilus Humans, Male, Media, Microbial, South Techniques, ducreyi,}, month = oct, note = {{PMID:} 1486890}, number = 10, pages = {930--934}, timestamp = {2011-03-11T10:06:07.000+0100}, title = {A simple medium for the primary isolation of Haemophilus ducreyi}, url = {http://www.ncbi.nlm.nih.gov/pubmed/1486890}, volume = 11, year = 1992 }