Abstract
The purpose of the study was to describe a convenient, reliable and
quantitative in vitro assay system to assess the cytocompatibility
of a calcium sulfate bone filler on two osteogenic cell lines and
primary osteoblasts. The hypothesis was that the bone void filler,
OsteoSet pellets, would not impact adversely on cell proliferation
kinetics or osteogenic potential of selected cells. The hypothesis
was tested by standard in vitro methodology of placing OsteoSet pellets
either directly in contact with osteogenic cells, or by compartmentalizing
within transwell - clear microporous membrane inserts. Data analyses
were accomplished with appropriate post hoc statistics (p < or =
0.05). In the presence of the OsteoSet pellets, the cell lines exhibited
a decrease in cell proliferation at days 4 and 7, independent of
either cell type or tissue culture medium. A decrease in the alkaline
phosphatase enzyme activity occurred in the osteogenic cell lines
maintained for 9 and 16 days in the presence of the OsteoSet pellets.
However, with the exception of the MC3T3E-1 line, no differences
were observed with respect to calcium deposition (mineralization)
by day 16. Intact human osteocalcin release data for the human-derived
OPC1 line and the primary osteoblasts was inconclusive as the OsteoSet
pellets may interact with the osteocalcin secreted into the tissue
culture medium. The present studies describe a cell culture system
to assess the cytocompatibility of bone-graft substitutes with osteogenic
cells by compartmentalizing material from direct cell contact (in
transwells), and additionally, by evaluating direct cell/biomaterial
interactions.
Users
Please
log in to take part in the discussion (add own reviews or comments).